{"created":"2023-07-27T06:38:32.831224+00:00","id":26649,"links":{},"metadata":{"_buckets":{"deposit":"e8e6ea22-ff79-4b90-8529-9cf6315ba309"},"_deposit":{"created_by":3,"id":"26649","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"26649"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00026649","sets":["1761:1762:1763"]},"author_link":["44784","22059","22233","44783","44782","44786","26700","44785","44781"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2045","bibliographicPageStart":"2040","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The stromal MC3T3-G2/PA6 (PA6) cells from mouse clavaria did not require insulin for differentiation into mature adipose cells, although insulin is well known to play a key role in adipocyte differentiation. Large lipid droplets were observed in the cytoplasm of PA6 cells, and mRNA expression of the adipose specific proteins (aP2, PPARγ, C/EBPα, FAS, GLUT4, leptin, and adiponectin) as differentiation markers appeared or increased clearly in the cells at 8 d after stimulation without insulin. In addition, the glycerol released from the cells (lipolysis) was increased in a concentration-dependent manner by isoproterenol. However, the isoproterenol-induced lipolysis in the cells was not influenced by treatment with insulin, although that was observed in extramedullary adipocytes, 3T3-L1 cells. On the other hand, the 2-deoxy-D-[1-3H]glucose uptake in differentiated PA6 cells also increased by insulin, as shown in other adipose cells. In the cells, insulin induced the phosphorylation of extracellular signal-regulated kinases (Erks), Akt at Ser 473 and ribosomal p70 S6 protein kinase (p70 S6K) at Thr 389, and the insulin-induced 2-deoxy-D-[1-3H]glucose uptake was inhibited by pre-treatment with wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), or ML-9, an Akt inhibitor. These results suggest that the insulin signal for adipogenesis (lipogenesis) and lipolysis in bone marrow stroma PA6 cells differs from extramedullary adipocytes, such as 3T3-L1 cells. © 2005 Pharmaceutical Society of Japan.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部附属病院薬剤部","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.28.2040","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jstage.jst.go.jp/article/bpb/28/11/28_2040/_article","subitem_relation_type_select":"URI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0918-6158","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Morita, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yaguchi, Kuniko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imada, Mikiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tachikawa, Chihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nomura, Masaaki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Moritani, Shuzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Manabu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokogawa, Koichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Miyamoto, Kenichi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"HO-PR-YOKOGWA-K-2040.pdf","filesize":[{"value":"732.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HO-PR-YOKOGWA-K-2040.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/26649/files/HO-PR-YOKOGWA-K-2040.pdf"},"version_id":"7b983737-2bfb-4a79-9cb4-37c3f56b9626"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Insulin signaling in adipocytes differentiated from mouse stromal MC3T3-G2/PA6 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Insulin signaling in adipocytes differentiated from mouse stromal MC3T3-G2/PA6 cells"}]},"item_type_id":"4","owner":"3","path":["1763"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"26649","relation_version_is_last":true,"title":["Insulin signaling in adipocytes differentiated from mouse stromal MC3T3-G2/PA6 cells"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-27T21:21:06.696990+00:00"}