{"created":"2023-07-27T06:38:33.358983+00:00","id":26661,"links":{},"metadata":{"_buckets":{"deposit":"2e10c0dc-b4f6-4205-9497-600ab092df58"},"_deposit":{"created_by":3,"id":"26661","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"26661"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00026661","sets":["1761:1762:1763"]},"author_link":["44829","20454","44830","44828","44831","44832","97"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-06-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"967","bibliographicPageStart":"955","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Prostate"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND. Although paclitaxel is used for hormone-resistant prostate cancer, relapse definitely occurs later. Details of the molecular mechanism responsible for paclitaxel- resistance remain unclear. METHODS. We established paclitaxel-resistant cells, DU145-TxR and PC-3-TxR from parent DU145 and PC-3. To characterize these cells, we examined cross-resistance to other anticancer drugs. Expression of several potential genes that had been related to drug-resistance was compared with parent cells by RT-PCR and Western blotting. Methylation analysis of multiple drug resistance (MDR1) promoter was carried out using bisulfite-modified DNA from cell lines. Knockdown experiments using small interfering RNA (siRNA) were also performed to confirm responsibility of drug-resistance. Finally, cDNA microarray was performed to quantify gene expression in PC-3 and PC-3-TxR cells. RESULTS. The IC50 for paclitaxel in DU145-TxR and PC-3-TxR was 34.0- and 43.4-fold higher than that in both parent cells, respectively. Both cells showed cross-resistance to some drugs, but not to VP-16 and cisplatin. Methylation analysis revealed that methylated CpG sites of MDR1 promoter in DU145 and PC-3 cells were demethylated in DU145-TxR cells, but not in PC-3-TxR cells. Knockdown of P-glycoprotein (P-gp), which was up-regulated in resistant cells, by MDR-1 siRNA restored paclitaxel sensitivity in DU145-TxR but not in PC-3-TxR, indicating that upregulation of P-gp was not always main cause of paclitaxel-resistance. Microarray analysis identified 201 (1.34%) up-regulated genes and 218 (1.45%) out of screened genes in PC-3-TxR. CONCLUSIONS. Our data will provide molecular mechanisms of paclitaxel-resistance and be useful for screening target genes to diagnose paclitaxel sensitivity. © 2007 Wiley-Liss, Inc.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部附属病院泌尿器科","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/pros.20581","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0270-4137","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeda, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizokami, Atsushi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Mamiya, Kiminori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Li, You Qiang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhang, Jian"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Keller, Evan T."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Namiki, Mikio"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"HO-PR-MIZOKAMI-A-955.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HO-PR-MIZOKAMI-A-955.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/26661/files/HO-PR-MIZOKAMI-A-955.pdf"},"version_id":"ee92cef2-e997-4000-9597-c0ceaa5e33d0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The establishment of two paclitaxel-resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The establishment of two paclitaxel-resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines"}]},"item_type_id":"4","owner":"3","path":["1763"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"26661","relation_version_is_last":true,"title":["The establishment of two paclitaxel-resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T12:24:19.227649+00:00"}