{"created":"2023-07-27T06:38:38.112648+00:00","id":26769,"links":{},"metadata":{"_buckets":{"deposit":"00a3d571-9537-476f-a219-bafa386e2db7"},"_deposit":{"created_by":3,"id":"26769","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"26769"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00026769","sets":["1761:1762:1763"]},"author_link":["45196","381","480","22694"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"5932","bibliographicPageStart":"5928","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Investigative ophthalmology & visual science"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ion channels in the ciliary epithelium play critical roles in the formation of aqueous humor in the eye. The present study identified a novel, swelling-activated K(+) current in freshly dissociated porcine pigmented ciliary epithelial cells. Ciliary epithelial cells were freshly dissociated from porcine eyes. Whole-cell currents were recorded by the patch-clamp technique in pigmented and nonpigmented ciliary epithelial cell (PCE-NPCE) pairs or single PCE cells. The 0-current potential was -49 ± 13 mV in PCE-NPCE cell pairs (n = 97) and -52 ± 12 mV in single PCE cells (n = 30). Whole-cell currents in these cells were dominated by an outwardly rectifying K(+) current activated by potentials more positive than -90 mV, which never inactivated during prolonged depolarization. The K(+) current was significantly augmented by hypotonic cell perfusion. External Ba(2+) was a blocker of this K(+) conductance (IC(50) of 0.38 mM), but the conductance was insensitive to external TEA(+). Linopirdine, a specific inhibitor of KCNQ channels, effectively blocked the K(+) current in these PCE cells. Porcine PCE cells express a swelling-activated K(+) channel, which may be a member of the KCNQ/Kv7 channel family. This K(+) channel is active near resting potentials and could contribute to the regulation of cell volume and water transport via the ciliary epithelia.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Association for Research in Vision and Ophthalmology (ARVO)"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1167/iovs.11-7173","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.iovs.org/","subitem_relation_type_select":"URI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1552-5783","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahira, Masayuki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Sakurai, Mayumi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Sakurada, Norimasa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sugiyama, Kazuhisa"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"HO-PR-TAKAHIRA-M-5928.pdf","filesize":[{"value":"446.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HO-PR-TAKAHIRA-M-5928.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/26769/files/HO-PR-TAKAHIRA-M-5928.pdf"},"version_id":"90e63b6a-5293-45c5-a317-71deb1094f11"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Swelling-activated potassium channel in porcine pigmented ciliary epithelial cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Swelling-activated potassium channel in porcine pigmented ciliary epithelial cells."}]},"item_type_id":"4","owner":"3","path":["1763"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"26769","relation_version_is_last":true,"title":["Swelling-activated potassium channel in porcine pigmented ciliary epithelial cells."],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T12:21:46.027051+00:00"}