@article{oai:kanazawa-u.repo.nii.ac.jp:00026804,
 author = {瀧, 淳一 and 若林, 大志 and 稲木, 杏吏 and 小川, 数馬 and 柴, 和弘 and 絹谷, 清剛 and Taki, Junichi and Wakabayashi, Hiroshi and Inaki, Anri and Imanaka-Yoshida, Kyoko and Hiroe, Michiaki and Ogawa, Kazuma and Morooka, Miyako and Kubota, Kazuo and Shiba, Kazuhiro and Yoshida, Toshimichi and Kinuya, Seigo},
 issue = {3},
 journal = {Journal of Nuclear Medicine},
 month = {Mar},
 note = {A relationship between L-[methyl-11C]methionine (11C-methionine) uptake and angiogenesis has been suggested in gliomas. However, methionine uptake in myocardial ischemia and reperfusion has received little attention. We investigated the serial changes and mechanisms of 14-Cmethionine uptake in a rat model of myocardial ischemia and reperfusion. Methods: The left coronary artery was occluded for 30 min, followed by reperfusion for 1-28 d. At the time of the study, 14-Cmethionine (0.74 MBq) and 201Tl (14.8 MBq) were injected intravenously at 20 and 10 min before sacrifice, respectively. One minute before sacrifice, the left coronary artery was reoccluded, and 99mTc-hexakis-2-methoxyisobutylisonitrile (150-180 MBq) was injected to verify the area at risk. Histologic sections of the heart were immunohistochemically analyzed using anti-CD68, anti-smooth-muscle a-actin (SMA), and antitroponin I and compared with the autoradiography findings. Results: Both 14Cmethionine (uptake ratio, 0.71 ± 0.13) and 201Tl uptake were reduced in the area at risk at 1 d after reperfusion. However, 3 d after reperfusion, an increased 14-Cmethionine uptake (1.79 ± 0.23) was observed corresponding to the area of still-reduced 201Tl uptake, and the 14-Cmethionine uptake gradually declined until 28 d. The increased 14-Cmethionine uptake area at 3 and 7 d corresponded well to the macrophage infiltrations demonstrated by positive CD68 staining. Anti-SMA staining appeared at 7 d, after which CD68 staining was gradually replaced by the SMA staining, suggesting that methionine uptake in the early phase after ischemia and reperfusion might reflect inflammatory activity. Conclusion: 14-Cmethionine accumulated in the infarcted area, and its uptake corresponded closely to macrophage infiltration at 3-7 d after reperfusion. Methionine imaging may be useful for inflammatory imaging early after myocardial infarction. COPYRIGHT © 2013 by the Society of Nuclear Medicine and Molecular Imaging, Inc., 金沢大学新学術創成研究機構 / 金沢大学附属病院},
 pages = {431--436},
 title = {14-Cmethionine uptake as a potential marker of inflammatory processes after myocardial ischemia and reperfusion},
 volume = {54},
 year = {2013}
}