@article{oai:kanazawa-u.repo.nii.ac.jp:00026818,
 author = {太田, 哲生 and 中川原, 寿俊 and 荒川, 元 and 二上, 文夫 and 月岡, 雄治 and 北川, 裕久 and 萱原, 正都 and 永川, 宅和 and 宮崎, 逸夫},
 issue = {4},
 journal = {日本消化器外科学会雑誌 = The Japanese journal of gastroenterological surgery},
 month = {Apr},
 note = {ヒト膵癌細胞3株 (Capan-1, BxPC-3, MIAPaCa-2) における serine protease の発現からみた肝転移能並びに protease inhibitor である FOY-305 投与による肝転移抑制効果をヌードマウスを用いた脾内移植法による肝転移モデル実験で評価した. さらに, vacuolar type proton pump (V-type ATPase)発現の有無を検索し, その inhibitor である bafilomycin A_1 による浸潤抑制効果を matrigel invasion chamber を用いた chemoinvasion assay 法で評価した. その結果, 膵癌の転移能は trypsinogen 1(cationic type) の発現とよく相関し, その転移発生率は FOY-305 投与で有意に抑制された. さらに, 膵癌の浸潤能は bafilomycin A_1 によって有意に抑制された. 以上の成績より, ヒト膵癌の浸潤・転移に際し trypsinogen 1 と V-type ATPase 発現が重要な役割を演じていること, さらに浸潤・転移の制御法として protease および proton pumpのinhibitor を用いた治療法の有用性が示唆された. The potential for hepatic metastasis in nude mice was studied by the intrasplenic implatation method with three human pancreatic cancer cell lines, Capan-1, BxPC-3, and MIAPaCa-2, especially in relation to serine protease expression. The inhibitory effect of a protease inhibitor agent, FOY-305, on hepatic metastasis was also assessed. In addition, we examined these cell lines, for expression of he vacuolar type proton pump (V-type ATPase), and determined the effect of its inhibitor agent, bafilomycin A1, on the cancer cell invasion in vitro by chemoinvasion assay using a MATRIGEL invasion chamber.The potential for hepatic metastasis was well correlated with expression of trypsinogen l (cationic isoform) in these cell lines, and the incidence of metastasis was significantly decreased by FOY-305. In addition, the potential for invasion in vitro in these cell lines was also siginificantly suppressed by bafilomycin A1. These finding suggest that pharmacologic inhibition of the activity of serine protease and V-type ATPase may be a new strategy for the therapy of pancreatic cancer invasion and metastasis.},
 pages = {888--892},
 title = {Protease inhibitor および proton pump inhibitor を用いた膵癌治療に対する新しい制癌治療戦略},
 volume = {29},
 year = {1996}
}