{"created":"2023-07-27T06:38:53.194479+00:00","id":27118,"links":{},"metadata":{"_buckets":{"deposit":"a8ca2687-5982-4cde-bb2e-f999b4e9cfd2"},"_deposit":{"created_by":3,"id":"27118","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27118"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027118","sets":["1761:1762:1763"]},"author_link":["93","46128","21405","20075","929","21299","660","108","569"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"4697","bibliographicPageStart":"4687","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"Cancer Research"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recent evidence suggests that a certain type of hepatocellular carcinoma (HCC) is hierarchically organized by a subset of cells with stem cell features (cancer stem cells; CSC). Although normal stem cells and CSCs are considered to share similar self-renewal programs, it remains unclear whether differentiation programs are also maintained in CSCs and effectively used for tumor eradication. In this study, we investigated the effect of oncostatin M (OSM), an interleukin 6-related cytokine known to induce the differentiation of hepatoblasts into hepatocytes, on liver CSCs. OSM receptor expression was detected in the majority of epithelial cell adhesion molecule-positive (EpCAM+) HCC with stem/progenitor cell features. OSM treatment resulted in the induction of hepatocytic differentiation of EpCAM+ HCC cells by inducing signal transducer and activator of transcription 3 activation, as determined by a decrease in stemness-related gene expression, a decrease in EpCAM, α-fetoprotein and cytokeratin 19 protein expressions, and an increase in albumin protein expression. OSM-treated EpCAM+ HCC cells showed enhanced cell proliferation with expansion of the EpCAM-negative non-CSC population. Noticeably, combination of OSM treatment with the chemotherapeutic agent 5-fluorouracil (5-FU), which eradicates EpCAM-negative non-CSCs, dramatically increased the number of apoptotic cells in vitro and suppressed tumor growth in vivo compared with either saline control, OSM, or 5-FU treatment alone. Taken together, our data suggest that OSM could be effectively used for the differentiation and active cell division of dormant EpCAM+ liver CSCs, and the combination of OSM and conventional chemotherapy with 5-FU efficiently eliminates HCC by targeting both CSCs and non-CSCs. ©2010 AACR.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院消化器内科","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Association for Cancer Research"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1158/0008-5472.CAN-09-4210","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00598557","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0008-5472","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamashita, Taro"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Honda, Masao"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Nio, Kouki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamoto, Yasunari"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Yamashita, Tatsuya"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Takamura, Hiroyuki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Tani, Takashi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Zen, Yoh"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kaneko, Shuichi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"HO-PR-YAMASHITA-T-4687.pdf","filesize":[{"value":"916.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HO-PR-YAMASHITA-T-4687.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27118/files/HO-PR-YAMASHITA-T-4687.pdf"},"version_id":"f3bee52d-34bc-47d4-916c-8934ec610421"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Oncostatin M renders epithelial cell adhesion molecule-positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Oncostatin M renders epithelial cell adhesion molecule-positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation"}]},"item_type_id":"4","owner":"3","path":["1763"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27118","relation_version_is_last":true,"title":["Oncostatin M renders epithelial cell adhesion molecule-positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T15:21:36.427740+00:00"}