{"created":"2023-07-27T06:39:04.020517+00:00","id":27368,"links":{},"metadata":{"_buckets":{"deposit":"3c2589f6-4ac7-4b7f-8777-a2b49f7fa8a7"},"_deposit":{"created_by":3,"id":"27368","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27368"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027368","sets":["1777:1778:1779"]},"author_link":["124","15557","15561","2781","96268","2907","47462","2999","23232","47461"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"166","bibliographicPageStart":"160","bibliographicVolumeNumber":"304","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"滝野, 隆久 "}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"宮森, 久志"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"清木, 元治 "}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"佐藤, 博"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Membrane-type 1 matrix metalloproteinase (MT1-MMP) is known to be internalized from cell surface, however, the fate of internalized MT1-MMP is still unknown. Here we demonstrate that at least a part of internalized MT1-MMP is targeted for lysosomal proteolysis. Treatment with an inhibitor of lysosomal proteinases chloroquine suppressed degradation of internalized MT1-MMP and induced accumulation of MT1-MMP in CD63-positive lysosomes. Ectopic expression of CD63 accelerated degradation of MT1-MMP, which was blocked by chloroquine. MT1-MMP, and CD63 were shown to form a complex through hemopexin-like domain of MT1-MMP and N-terminal region of CD63, and thus accelerated degradation of MT1-MMP was not observed with mutants lacking these domains. CD63 mutant lacking lysosomal targeting motif was unable to promote MT1-MMP degradation. These results suggest that CD63 regulates MT1-MMP by targeting to lysosomes.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00027355","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/s0006-291x(03)00544-8","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/0006291X","subitem_relation_type_select":"URI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takino, Takahisa"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Miyamori, Hisashi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kawaguchi, Noriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uekita, Takamasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seiki, Motoharu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sato, Hiroshi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-TAKINO-T-Takino[1].BBRC.pdf","filesize":[{"value":"420.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-TAKINO-T-Takino[1].BBRC.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27368/files/CA-PR-TAKINO-T-Takino[1].BBRC.pdf"},"version_id":"834e8d5d-2ad3-4ee1-ae5c-66c4c2be6b0a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Tetraspanin CD63 Promotes Targetion and Lysosomal Proteolysis of Membrance-Type 1 Matrix Metalloproteinase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tetraspanin CD63 Promotes Targetion and Lysosomal Proteolysis of Membrance-Type 1 Matrix Metalloproteinase"}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27368","relation_version_is_last":true,"title":["Tetraspanin CD63 Promotes Targetion and Lysosomal Proteolysis of Membrance-Type 1 Matrix Metalloproteinase"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T11:06:33.145477+00:00"}