{"created":"2023-07-27T06:39:04.192302+00:00","id":27372,"links":{},"metadata":{"_buckets":{"deposit":"167515a1-1fa8-4951-8780-5d7bbf9df298"},"_deposit":{"created_by":3,"id":"27372","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27372"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027372","sets":["1777:1778:1779"]},"author_link":["47486","47484","27970","47485","256","83","478"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"1756","bibliographicPageStart":"1748","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Oncogene Volume"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今村, 龍"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"木下, 健"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"須田, 貴司"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Apoptosis-associated speck-like protein containing a CARD (ASC) is an adaptor molecule that mediates apoptotic and inflammatory signals, and implicated in tumor suppression. However, the mechanism of ASC-mediated apoptosis has not been well elucidated. Here, we investigated the molecular mechanisms of ASC-mediated apoptosis in several cell lines using a caspase recruitment domain 12-Nod2 chimeric protein that transduces the signal from muramyl dipeptide into ASC-mediated apoptosis. Experiments using dominant-negative mutants, small-interfering RNAs and peptide inhibitors for caspases indicated that caspase-8 was generally required for ASC-mediated apoptosis, whereas a requirement for caspase-9 depended on the cell type. In addition, caspase-like apoptosis-regulatory protein (CLARP)/Fas-like inhibitor protein, a natural caspase-8 inhibitor, suppressed ASC-mediated apoptosis, and Clarp-/- mouse embryonic fibroblasts were highly sensitive to ASC-mediated apoptosis. Bax-deficient HCT116 cells were resistant to ASC-mediated apoptosis as reported previously, although we failed to observe colocalization of ASC and Bax in cells. Like Fas-ligand-induced apoptosis, the ASC-mediated apoptosis was inhibited by Bcl-2 and/or Bcl-XL in type-II but not type-I cell lines. Bid was cleaved upon ASC activation, and suppression of endogenous Bid expression using small-interfering RNAs in type-II cells reduced the ASC-mediated apoptosis. These results indicate that ASC, like death receptors, mediates two types of apoptosis depending on the cell type, in a manner involving caspase-8. © 2007 Nature Publishing Group All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所がん病態制御","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00027359","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/sj.onc.1209965","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10687380","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0950-9232","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Mizuho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawase, Kouji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inohara, Naohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imamura, Ryu"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yeh, Wen-Chen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kinoshita, Takeshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Suda, Takashi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-SUDA-T-1748.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-SUDA-T-1748.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27372/files/CA-PR-SUDA-T-1748.pdf"},"version_id":"508369ec-0919-4af7-a1f8-c454fd1e4be8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mechanism of ASC-mediated apoptosis: Bid-dependent apoptosis in type II cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mechanism of ASC-mediated apoptosis: Bid-dependent apoptosis in type II cells"}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27372","relation_version_is_last":true,"title":["Mechanism of ASC-mediated apoptosis: Bid-dependent apoptosis in type II cells"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T11:06:24.163297+00:00"}