{"created":"2023-07-27T06:39:04.883772+00:00","id":27388,"links":{},"metadata":{"_buckets":{"deposit":"4310001e-eab0-451b-9084-a03a83524174"},"_deposit":{"created_by":3,"id":"27388","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27388"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027388","sets":["1777:1778:1779"]},"author_link":["47535","47537","47538","47536","27","47539","47534"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"485","bibliographicPageStart":"478","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"Journal of Biotechnology"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"松本, 邦夫","creatorNameLang":""},{"creatorName":"マツモト, クニオ","creatorNameLang":""}],"familyNames":[{"familyName":"松本","familyNameLang":""},{"familyName":"マツモト","familyNameLang":""}],"givenNames":[{"givenName":"邦夫","givenNameLang":""},{"givenName":"クニオ","givenNameLang":""}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatocyte growth factor (HGF) is biosynthesized as a biologically inactive, single-chain form (pro-HGF). Its activation is associated with cleavage at Arg494-Val495 into a two-chain mature form composed of disulfide-linked α- and β-chains. Because serum is a major source of HGF activator (the predominant serine protease responsible for the processing of pro-HGF), serum-free production of recombinant, two-chain HGF had not been established. In this study, to enable serum-free production of two-chain HGF, we generated engineered human pro-HGFs that can be specifically cleaved and activated by Genenase I. Since Genenase I specifically cleaves the C-terminus of the His-Tyr sequence, which does not exist in human HGF, Arg494 (the C-terminus of the HGF α-chain) was replaced by His-Tyr, Ala-Ala-His-Tyr, Pro-Gly-His-Tyr, or Pro-Gly-Ala-Ala-His-Tyr. Genenase I cleaved engineered pro-HGFs specifically at the replaced amino acid sequences, forming a disulfide-linked two-chain form. The cleavage was most efficient in the case of the Pro-Gly-Ala-Ala-His-Tyr sequence, and cleaved HGFs displayed biological activities identical to those of wild-type HGF. Considering a potential medical application of HGF, the present technique is valuable because it enables the production of recombinant, two-chain HGF entirely without serum and extends the choice of host cells and organisms for recombinant production. © 2007 Elsevier B.V. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所附属分子標的がん医療研究開発センター","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00027375","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jbiotec.2007.11.006","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10458361","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0168-1656","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayata, Daichika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuta, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"松本, 邦夫","creatorNameLang":""},{"creatorName":"マツモト, クニオ","creatorNameLang":""}],"familyNames":[{"familyName":"松本","familyNameLang":""},{"familyName":"マツモト","familyNameLang":""}],"givenNames":[{"givenName":"邦夫","givenNameLang":""},{"givenName":"クニオ","givenNameLang":""}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Adachi, Eri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hanada, Keigo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Adachi, Kiichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Toshikazu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-MATSUMOTO-K-478.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-MATSUMOTO-K-478.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27388/files/CA-PR-MATSUMOTO-K-478.pdf"},"version_id":"264db8ba-78dd-4149-94e0-db1c7d6c8539"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I"}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27388","relation_version_is_last":true,"title":["Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-05-13T06:08:35.917251+00:00"}