{"created":"2023-07-27T06:39:05.490230+00:00","id":27402,"links":{},"metadata":{"_buckets":{"deposit":"a61e8983-9c1a-40bc-a6b3-6cee3ef2b7ad"},"_deposit":{"created_by":3,"id":"27402","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27402"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027402","sets":["1777:1778:1779"]},"author_link":["1818","47617","302","47615","47616","256","83","478"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-06-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"7662","bibliographicPageStart":"7655","bibliographicVolumeNumber":"182","bibliographic_titles":[{"bibliographic_title":"Journal of immunology"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今村, 龍"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"西内, 巧"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"木下, 健"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"須田, 貴司"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is an adaptor molecule that mediates inflammatory and apoptotic signals. Although the role of ASC in caspase-1-mediated IL-1beta and IL-18 maturation is well known, ASC also induces NF-kappaB activation and cytokine gene expression in human cells. In this study, we investigated the molecular mechanism and repertoire of ASC-induced gene expression in human cells. We found that the specific activation of ASC induced AP-1 activity, which was required for optimal IL8 promoter activity. ASC activation also induced STAT3-, but not STAT1-, IFN-stimulated gene factor 3- or NF-AT-dependent reporter gene expression. The ASC-mediated AP-1 activation was NF-kappaB-independent and primarily cell-autonomous response, whereas the STAT3 activation required NF-kappaB activation and was mediated by a factor that can act in a paracrine manner. ASC-mediated AP-1 activation was inhibited by chemical or protein inhibitors for caspase-8, caspase-8-targeting small-interfering RNA, and p38 and JNK inhibitors, but not by a caspase-1 inhibitor, caspase-9 or Fas-associated death domain protein (FADD) dominant-negative mutants, FADD- or RICK-targeting small-interfering RNAs, or a MEK inhibitor, indicating that the ASC-induced AP-1 activation is mediated by caspase-8, p38, and JNK, but does not require caspase-1, caspase-9, FADD, RICK, or ERK. DNA microarray analyses identified 75 genes that were induced by ASC activation. A large proportion of them was related to transcription (23%), inflammation (21%), or cell death (16%), indicating that ASC is a potent inducer of inflammatory and cell death-related genes. This is the first report of ASC-mediated AP-1 activation and the repertoire of genes induced downstream of ASC activation.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00027389","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Association of Immunologists"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.4049/jimmunol.0800448","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00699656","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-1767","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Mizuho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imamura, Ryu"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Motani, Kou"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishiuchi, Takumi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Matsumoto, Norihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kinoshita, Takeshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Suda, Takashi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-SUDA-T-7655.pdf","filesize":[{"value":"101.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-SUDA-T-7655.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27402/files/CA-PR-SUDA-T-7655.pdf"},"version_id":"c00d625d-b767-46cc-a590-11aa14caf5cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mechanism and repertoire of ASC-mediated gene expression","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mechanism and repertoire of ASC-mediated gene expression"}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27402","relation_version_is_last":true,"title":["Mechanism and repertoire of ASC-mediated gene expression"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:50:55.484634+00:00"}