{"created":"2023-07-27T06:39:05.576843+00:00","id":27404,"links":{},"metadata":{"_buckets":{"deposit":"dde32191-ded6-41ea-82c7-1d31fb3802ea"},"_deposit":{"created_by":3,"id":"27404","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27404"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027404","sets":["1777:1778:1779"]},"author_link":["21362","65","21711","47622"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1602","bibliographicPageStart":"1597","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"World Journal of Gastroenterology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aim: To investigate the expression of genes involved in the gemcitabine-induced cytotoxicity in human pancreatic cancer cells. Methods: A human pancreatic cancer cell line, PANC-1, was cultured. 1 × 104 PANC-1 cells were plated in 96-well microtiter plates. After being incubated for 24 h, gemcitabine was added to the medium at concentrations ranging 2.5 -1 000 mg/L. The AlamarBlue dye method was used for cell growth analysis. DNA fragmentation was quantitatively assayed using a DNA fragmentation enzyme-linked immunosorbent assay (ELISA) kit. PAP and TP53INP1 mRNA expression was determined using the reverse transcription-polymerase chain reaction with semi-quantitative analysis. The expression of GSK-3β and phospho-GSK-3β proteins was examined with Western blot analysis. Results: The IC50 for the drug after a 48-h exposure to gemcitabine was 16 mg/L. The growth of PANC-1 cells was inhibited by gemcitabine in a concentration-dependent manner (P< 0.0001) and the cell growth was also inhibited throughout the time course (P<0.0001). The DNA fragmentation rate in the gemcitabine-treated group at 48 h was 44.7 %, whereas it was 25.3 % in the untreated group. The PAP mRNA expression was decreased after being treated with gemcitabine, whereas the TP53INP1 mRNA was increased by the gemcitabine treatment. Western blot analysis showed that phospho-GSK-3βser9 was induced by the gemcitabine treatment. Conclusion: Gemcitabine suppresses PANC-1 cell proliferation and induces apoptosis. Apoptosis is considered to be associated with the inhibition of PAP and GSK-3β, and the activation of TP53INP1 and pospho-GSK-3βser9. © 2006 The WJG Press. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"WJG Press"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3748/wjg.v12.i10.1597","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11690631","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1007-9327","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jiang, Pei-Hong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Motoo, Yoshiharu"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Sawabu, Norio"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Minamoto, Toshinari"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-MINAMOTO-T-1597.pdf","filesize":[{"value":"365.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CA-PR-MINAMOTO-T-1597.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27404/files/CA-PR-MINAMOTO-T-1597.pdf"},"version_id":"bd228e6f-3a49-408b-877a-1064f878f21c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of gemcitabine on the expression of apoptosis-related genes in human pancreatic cancer cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of gemcitabine on the expression of apoptosis-related genes in human pancreatic cancer cells."}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27404","relation_version_is_last":true,"title":["Effect of gemcitabine on the expression of apoptosis-related genes in human pancreatic cancer cells."],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T11:00:58.898060+00:00"}