@article{oai:kanazawa-u.repo.nii.ac.jp:00027409,
 author = {Motoo, Yoshiharu and Xie, Min-Jue and Su, Shi-Bing and Sawabu, Norio},
 issue = {4},
 journal = {Journal of traditional medicines = 和漢医薬学雑誌},
 month = {Aug},
 note = {柴胡桂枝湯(TJ-10)は慢性膵炎の経口治療薬として臨床的に使用されてきた。本総説ではWBN/Kobラットにおける自然発症慢性膵炎に対するTJ-10の治療効果の分子機構について我々の実験結果を中心に述べた。 RT-PCR解析によるpancreatitis-associated protein(PAP)やサイトカイン(TNF-α,IL-6など)の発現抑制からTJ-10の抗炎症作用が示唆された。アザン染色からみた膵線維化改善やTGF-β,α平滑筋アクチン,III型コラーゲン,ファイブロネクチンなどの線維化関連因子の発現抑制によりTJ-10の抗線維化作用を確認した。 TUNEL法やFas,Fasリガンドなどのアポトーシス関連因子の発現抑制より膵腺房細胞に対するTJ-10の抗アポトーシス作用が示唆された。最後にSODの発現増加とiNOS発現抑制によりTJ-10の抗酸化作用を確認した。以上より,TJ-10は抗炎症・抗線維化・抗アポトーシス・抗酸化の4つの作用機序により慢性膵炎に有効であると考えられた。Saiko-keishi-to (TJ-10) has been clinically used as an oral therapeutic drug for chronic pancreatitis. In this review, we present our data on molecular action mechanisms of TJ-10 on spontaneous chronic pancreatitis in the WBN/Kob rats. Anti-inflammatory effects of TJ-10 were suggested by the suppression of the gene expressions of pancreatitis-associated protein (PAP), cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Anti-fibrotic effects were confirmed by the observations on the amelioration of pancreatic fibrosis assessed with AZAN staining and the analysis of expressions of fibrosis-related factors such as transforming growth factor (TGF)-β, α-smooth muscle actin, type III collagen and fibronectin. Anti-apoptotic effect was examined with the TUNEL method and the analysis of apoptosis-related factors such as Fas and Fas ligand. Anti-oxidant effect was suggested by the findings of TJ-10-induced enhancement of the expression of superoxide dismutase and the suppression of the expression of inducible nitric oxide synthase. These results suggest that TJ-10 is effective for chronic pancreatitis by the major four action mechanisms: anti-inflammatory, anti-fibrotic, anti-apoptotic, and anti-oxidant effects., 金沢大学がん研究所},
 pages = {143--149},
 title = {Molecular mechanisms of therapeutic effects of Saiko-keishi-to on spontaneous chronic pancreatitis in the WBN/Kob rat},
 volume = {20},
 year = {2003}
}