@article{oai:kanazawa-u.repo.nii.ac.jp:00027434, author = {Ogura, Hisashi and Sato, Hiroshi and Hatano, Motoichi}, issue = {2}, journal = {Journal of General Virology}, month = {Jan}, note = {HVJ isolated from culture fluids of G2, THEL and GM2 cells persistently infected with HVJ (G2-HVJ, THEL-HVJ and GM2-HVJ) were characterized in comparison with wild-type HVJ (HVJo). Viral structural proteins were analysed by 10% SDS-polyacrylamide gel electrophoresis and it was found that only the P polypeptides of all the HVJ clones isolated from G2-HVJ cell shad a smaller size mol. wt. 77000 (77K), than that of HVJo with a mol. wt. of 79000 (79K). One of six clones from THEL-HVJ cells and one of ten clones from GM2-HVJ cells exhibited the same migration pattern of P polypeptide as that of the clones from G2-HVJ cells. However, the other structural proteins were not different from those of the wild-type virions. All the clones from these carrier cultures were temperature-sensitive and were blocked in early step(s) required for RNA synthesis. These results indicate that some mutation(s) associated with P polypeptide could occur during the course of HVJ persistent infection in cell cultures.}, pages = {469--473}, title = {Temperature-sensitive HVJ (Sendai virus) with altered P polypeptide derived from persistently infected cell lines}, volume = {55}, year = {1981} }