@article{oai:kanazawa-u.repo.nii.ac.jp:00027463,
 author = {Uchimayashi, Tadao and Egawa, Masayuki and Asari, Toyoki and Nakajima, Kazuyoshi and Hisazumi, Haruo and Endo, Yoshio and Tanaka, Motohiro and Sasaki, Takuma and 打林, 忠雄 and 江川, 雅之 and 浅利, 豊紀 and 中嶋, 和喜 and 久住, 治男 and 遠藤, 良夫 and 田中, 基裕 and 佐々木, 琢磨},
 issue = {1},
 journal = {日本泌尿器科学会雑誌},
 month = {Jan},
 note = {継代培養細胞株 (B16-F10メラノーマ, ヒト膀胱癌由来培養細胞株KK-47) および臨床材料 (腎細胞癌5例, 尿路移行上皮癌9例) を用いて鶏卵法による温熱と抗癌剤併用効果の検討を行なった. その結果マウスB16-F10メラノーマではADR単独処理群およびCDDP+温熱, ADR+温熱, CY+温熱併用群において有意に高い腫瘍発育阻止率が認められた (P<0.05, P<0.01). KK-47ではCY単独処理群およびCY+温熱処理群において有意に高い腫瘍発育阻止率が認められた (P<0.05). 臨床材料を用いた検討では14例中11例 (79%) において感受性試験の評価が可能であり, 今後これらの結果を臨床応用し, その相関性につき検討していく予定である. The chorioallantoic membarane of chick embryo was used to examine the responses of tumor cell lines and surgically resected specimens to anticancer agents in combination with or without hyperthermia. B16-F10 melanoma, KK-47 cell line derived from a transitional cell carcinoma of the bladder and surgical specimens from urological malignancies were transplanted onto the chorioallantoic membrane of chick embryo 10 days after fertilization. The effects of intravascularlly injected cyclophosphamide, cisplatin, adriamycin, mitomycin C, vincristine and vinblastine, in combination with or without hyperthermia, on the growth of the grafts were investigated. A 15-minute 42.5°C hyperthermia exhibited most favorable anticancer effects in B16-F10 grafts (P<0.01) of all heating conditions tested. A combined use of hyperthermia and cisplatin, cyclophosphamide or adriamycin demonstrated a significantly high tumor regression rate in B16-F10 grafts (P<0.01). In KK-47 grafts, a single use of cyclophosphamide and a combined use of hyperthermia and cyclophosphamede exhibited a highly significant antitumor regression (P<0.05). Surgical specimens from 14 urological malignancies were subjected to this assay. Growth which was adequate for sensitivity test was obtained in 11 of the 14 malignancies. The results obtained suggest that this assay system may be a useful chemohyperthermia sensitivity test.},
 pages = {17--21},
 title = {鶏卵法による温熱と抗癌剤併用効果の検討},
 volume = {80},
 year = {1989}
}