{"created":"2023-07-27T06:39:10.694195+00:00","id":27521,"links":{},"metadata":{"_buckets":{"deposit":"f27bc8c0-7212-4af8-a128-d1b53cf4f1fd"},"_deposit":{"created_by":3,"id":"27521","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27521"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027521","sets":["1777:1778:1779"]},"author_link":["124","23232","282","47988"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"353","bibliographicPageStart":"347","bibliographicVolumeNumber":"108","bibliographic_titles":[{"bibliographic_title":"Cancer Science"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An artificial receptor for proMMP-9 was created by fusing tissue inhibitor of MMP-1 (TIMP-1) with type II transmembrane mosaic serine protease (MSP-T1). Expression of MSP-T1 in 293T cells induced binding of proMMP-9, which was processed by MMP-2 activated by membrane type 1 MMP (MT1-MMP). HT1080 cells transfected with the MSP-T1 gene produced activated MMP-9 in collagen gel, and addition of proMMP-2 to the culture augmented it, which resulted in intensive collagen digestion. These cells metastasized into chick embryonic liver more than control cells. Treatment of HT1080 cells with concanavalin A in the presence of exogenous proMMP-2 induced activation of not only proMMP-2 but also proMMP-9. Knockdown of MT1-MMP or TIMP-2 expression with siRNA suppressed activation of both proMMP-2 and proMMP-9. Transfection of TIMP-1 siRNA suppressed cell binding and activation of proMMP-9, but not proMMP-2 activation. Knockdown of a disintegrin and metalloproteinase 10 (ADAM10) expression reduced cell binding and processing of proMMP-9. These results suggest that proMMP-9, which binds to a receptor complex containing TIMP-1 and ADAM10, is activated by the MT1-MMP/MMP-2 axis, and MMP-9 thus activated stimulates cellular proteolysis and metastasis. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Cancer Association = 日本癌学会 / John Wiley & Sons"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/cas.13134","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jca.gr.jp/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291349-7006","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association."}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11808050","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-9032","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Li, Zichen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takino, Takahisa"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Endo, Yoshio"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Sato, Hiroshi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-SATO-H-347.pdf","filesize":[{"value":"907.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CA-PR-SATO-H-347.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27521/files/CA-PR-SATO-H-347.pdf"},"version_id":"797c9280-aa0b-420d-8085-f78de24f83e8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Activation of MMP-9 by membrane type-1 MMP/MMP-2 axis stimulates tumor metastasis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Activation of MMP-9 by membrane type-1 MMP/MMP-2 axis stimulates tumor metastasis"}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27521","relation_version_is_last":true,"title":["Activation of MMP-9 by membrane type-1 MMP/MMP-2 axis stimulates tumor metastasis"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-27T21:05:13.426233+00:00"}