| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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|
タイトル |
Deregulated GSK3β sustains gastrointestinal cancer cells survival by modulating human telomerase reverse transcriptase and telomerase |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.24517/00027533 |
|
ID登録タイプ |
JaLC |
| 著者 |
Mai, Wei
Kawakami, Kazuyuki
Shakoori, Abbas
Kyo, Satoru
Miyashita, Katsuyoshi
Yokoi, Kenji
Jin, Mingji
Shimasaki, Takeo
Motoo, Yoshiharu
Minamoto, Toshinari
|
| 著者別表示 |
麦, 威
川上, 和之
京, 哲
宮下 , 勝吉
金, 明姫
島崎, 猛夫
元雄, 良治
源, 利成
|
| 提供者所属 |
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|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学がん研究所分子標的がん医療研究開発センター |
| 書誌情報 |
Clinical Cancer Research
巻 15,
号 22,
p. 6810-6819,
発行日 2009-11-15
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1078-0432 |
| NCID |
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識別子タイプ |
NCID |
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|
関連識別子 |
BA34114081 |
| DOI |
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|
関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1158/1078-0432.CCR-09-0973 |
| 出版者 |
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出版者 |
American Association for Cancer Research |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Purpose: Glycogen synthase kinase-3β (GSK3β) regulates multiple cell signaling pathways and has been implicated in glucose intolerance, neurodegenerative disorders, and inflammation. We investigated the expression, activity, and putative pathologic role of GSK3β in gastrointestinal, pancreatic, and liver cancers. Experimental Design: Colon, stomach, pancreatic, and liver cancer cell lines; nonneoplastic HEK293 cells; and matched pairs of normal and tumor tissues of stomach and colon cancer patients were examined for GSK3β expression and its phosphorylation at serine 9 (inactive form) and tyrosine 216 (active form) by Western immunoblotting and for GSK3β activity by in vitro kinase assay. The effects of small-molecule GSK3β inhibitors and of RNA interference on cell survival, proliferation, and apoptosis were examined in vitro and on human colon cancer cell xenografts in athymic mice. The effects of GSK3β inhibition on human telomerase reverse transcriptase (hTERT) expression and telomerase activity were compared between colon cancer and HEK293 cells. Results: Cancer cell lines and most cancer tissues showed increased GSK3β expression and increased tyrosine 216 phosphorylation and activity but decreased serine 9 phosphorylation compared with HEK293 cells and nonneoplastic tissues. Inhibition of GSK3β resulted in attenuated cell survival and proliferation and increased apoptosis in most cancer cell lines and in HT-29 xenografts in rodents but not in HEK293 cells. GSK3β inhibition in colon cancer cells was associated with decreased hTERT expression and telomerase activity. Conclusion: The results indicate that deregulated GSK3β sustains gastrointestinal cancer cells survival through modulation of hTERT and telomerase. © 2009 American Association for Cancer Research. |
| 著者版フラグ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
CA-PR-MINAMOTO-T-6810.pdf (3.2 MB)
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