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Functional interaction of hepatitis C virus NS5B with nucleolin GAR domain
https://doi.org/10.24517/00027544
https://doi.org/10.24517/0002754467700d69-305c-4015-99b4-f3b006e17bea
| 名前 / ファイル | ライセンス | アクション |
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CA-PR-MURAKAMI-S-917.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2017-10-05 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Functional interaction of hepatitis C virus NS5B with nucleolin GAR domain | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| ID登録 | ||||||||||||||
| ID登録 | 10.24517/00027544 | |||||||||||||
| ID登録タイプ | JaLC | |||||||||||||
| 著者 |
Kusakawa, Takashi
× Kusakawa, Takashi× Shimakami, Tetsuro× Kaneko, Shuichi× Yoshioka, Katsuji× Murakami, Seishi |
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| 著者別表示 |
島上, 哲朗
× 島上, 哲朗
× 金子, 周一
× 善岡, 克次
× 村上, 清史
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| 提供者所属 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | 金沢大学がん研究所 | |||||||||||||
| 書誌情報 |
Journal of Biochemistry 巻 141, 号 6, p. 917-927, 発行日 2007-06-01 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0021-924X | |||||||||||||
| NCID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA00694073 | |||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1093/jb/mvm102 | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | 日本生化学会 = Japanese Biochemical Society | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Hepatitis C Virus (HCV) non-structural proteins are major components of replication complex that is modulated by several host factors. We previously reported that nucleolin, a representative nucleolar marker, interacts with the NS5B through two separated sequences, amino acids (aa) 208-214 and 500-506, and that W208 in the former stretch is essential for both nucleolin-binding and HCV replication. Here we evaluated the role of the latter stretch aa 500-506 of WRHRARS in nucleolin-binding and HCV replication scanned by alanine-substituted clustered mutant (cm) or point mutant (pm). One tryptophan and three arginine residues in the sequence were found to be essential both for nucleolin-binding in vivo and HCV replication detected with a HCV subgenomic replicon transfected into Huh7 cells. NS5B-binding of nucleolin was further delineated by truncation and clustered mutants of nucleolin. Arginine-glycine-glycine (RGG) repeat in the Glycine arginine rich (GAR) domain were defined to be indispensable for NS5B-binding immunologically detected in in vivo and in vitro although short internal-truncations of RGG repeat are tolerable for NS5B-binding. These results indicate that nucleolin is a critical host factor for HCV replication through the direct interaction between W208 and several residues at the sequence, aa 500-505, of NS5B, and the long-turn motif including RGG repeat at nucleolin C-terminal. © 2007 The Japanese Biochemical Society. | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | © 2007 The Japanese Biochemical Society. | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
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| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | http://dx.doi.org/10.1093/jb/mvm102 | |||||||||||||
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| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://jb.oxfordjournals.org/cgi/content/abstract/141/6/917 | |||||||||||||
