| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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|
タイトル |
Functional interaction of hepatitis C virus NS5B with nucleolin GAR domain |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.24517/00027544 |
|
ID登録タイプ |
JaLC |
| 著者 |
Kusakawa, Takashi
Shimakami, Tetsuro
Kaneko, Shuichi
Yoshioka, Katsuji
Murakami, Seishi
|
| 著者別表示 |
島上, 哲朗
金子, 周一
善岡, 克次
村上, 清史
|
| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学がん研究所 |
| 書誌情報 |
Journal of Biochemistry
巻 141,
号 6,
p. 917-927,
発行日 2007-06-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021-924X |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00694073 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/jb/mvm102 |
| 出版者 |
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出版者 |
日本生化学会 = Japanese Biochemical Society |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Hepatitis C Virus (HCV) non-structural proteins are major components of replication complex that is modulated by several host factors. We previously reported that nucleolin, a representative nucleolar marker, interacts with the NS5B through two separated sequences, amino acids (aa) 208-214 and 500-506, and that W208 in the former stretch is essential for both nucleolin-binding and HCV replication. Here we evaluated the role of the latter stretch aa 500-506 of WRHRARS in nucleolin-binding and HCV replication scanned by alanine-substituted clustered mutant (cm) or point mutant (pm). One tryptophan and three arginine residues in the sequence were found to be essential both for nucleolin-binding in vivo and HCV replication detected with a HCV subgenomic replicon transfected into Huh7 cells. NS5B-binding of nucleolin was further delineated by truncation and clustered mutants of nucleolin. Arginine-glycine-glycine (RGG) repeat in the Glycine arginine rich (GAR) domain were defined to be indispensable for NS5B-binding immunologically detected in in vivo and in vitro although short internal-truncations of RGG repeat are tolerable for NS5B-binding. These results indicate that nucleolin is a critical host factor for HCV replication through the direct interaction between W208 and several residues at the sequence, aa 500-505, of NS5B, and the long-turn motif including RGG repeat at nucleolin C-terminal. © 2007 The Japanese Biochemical Society. |
| 権利 |
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|
権利情報 |
© 2007 The Japanese Biochemical Society. |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連URI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
http://dx.doi.org/10.1093/jb/mvm102 |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
http://jb.oxfordjournals.org/cgi/content/abstract/141/6/917 |
CA-PR-MURAKAMI-S-917.pdf (1.1 MB)
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