{"created":"2023-07-27T06:39:12.245733+00:00","id":27557,"links":{},"metadata":{"_buckets":{"deposit":"4a7cd0e9-5229-4b99-b311-86223f657729"},"_deposit":{"created_by":3,"id":"27557","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27557"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027557","sets":["1777:1778:1779"]},"author_link":["142","48096","95536","62","550","26139","2674","24425","68205"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"927","bibliographicPageStart":"917","bibliographicVolumeNumber":"141","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"島上, 哲朗"}],"nameIdentifiers":[{"nameIdentifier":"550","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50436820","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=50436820"},{"nameIdentifier":"50436820","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050436820"}]},{"creatorNames":[{"creatorName":"金子, 周一"}],"nameIdentifiers":[{"nameIdentifier":"68205","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60185923","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60185923"}]},{"creatorNames":[{"creatorName":"善岡, 克次"}],"nameIdentifiers":[{"nameIdentifier":"2674","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60200937","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60200937"}]},{"creatorNames":[{"creatorName":"村上, 清史"}],"nameIdentifiers":[{"nameIdentifier":"95536","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90019878","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90019878"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatitis C Virus (HCV) non-structural proteins are major components of replication complex that is modulated by several host factors. We previously reported that nucleolin, a representative nucleolar marker, interacts with the NS5B through two separated sequences, amino acids (aa) 208-214 and 500-506, and that W208 in the former stretch is essential for both nucleolin-binding and HCV replication. Here we evaluated the role of the latter stretch aa 500-506 of WRHRARS in nucleolin-binding and HCV replication scanned by alanine-substituted clustered mutant (cm) or point mutant (pm). One tryptophan and three arginine residues in the sequence were found to be essential both for nucleolin-binding in vivo and HCV replication detected with a HCV subgenomic replicon transfected into Huh7 cells. NS5B-binding of nucleolin was further delineated by truncation and clustered mutants of nucleolin. Arginine-glycine-glycine (RGG) repeat in the Glycine arginine rich (GAR) domain were defined to be indispensable for NS5B-binding immunologically detected in in vivo and in vitro although short internal-truncations of RGG repeat are tolerable for NS5B-binding. These results indicate that nucleolin is a critical host factor for HCV replication through the direct interaction between W208 and several residues at the sequence, aa 500-505, of NS5B, and the long-turn motif including RGG repeat at nucleolin C-terminal. © 2007 The Japanese Biochemical Society.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00027544","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会 = Japanese Biochemical Society"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvm102","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1093/jb/mvm102","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://jb.oxfordjournals.org/cgi/content/abstract/141/6/917","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2007 The Japanese Biochemical Society."}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kusakawa, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"48096","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimakami, Tetsuro"}],"nameIdentifiers":[{"nameIdentifier":"26139","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50436820","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50436820"}]},{"creatorNames":[{"creatorName":"Kaneko, Shuichi"}],"nameIdentifiers":[{"nameIdentifier":"62","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60185923","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60185923"},{"nameIdentifier":"60185923","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=60185923"},{"nameIdentifier":"60185923","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060185923"}]},{"creatorNames":[{"creatorName":"Yoshioka, Katsuji"}],"nameIdentifiers":[{"nameIdentifier":"142","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60200937","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60200937"},{"nameIdentifier":"60200937","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=60200937"},{"nameIdentifier":"60200937","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060200937"}]},{"creatorNames":[{"creatorName":"Murakami, Seishi"}],"nameIdentifiers":[{"nameIdentifier":"24425","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90019878","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90019878"},{"nameIdentifier":"90019878","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090019878"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-MURAKAMI-S-917.pdf","filesize":[{"value":"1.1 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domain"}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27557","relation_version_is_last":true,"title":["Functional interaction of hepatitis C virus NS5B with nucleolin GAR domain"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-06-25T04:59:43.903175+00:00"}