{"created":"2023-07-27T06:39:12.288930+00:00","id":27558,"links":{},"metadata":{"_buckets":{"deposit":"e188765a-d52b-40be-992e-1982e69069db"},"_deposit":{"created_by":3,"id":"27558","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"27558"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00027558","sets":["1777:1778:1779"]},"author_link":["48099","20578","457","49","48098"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"404","bibliographicPageStart":"396","bibliographicVolumeNumber":"100","bibliographic_titles":[{"bibliographic_title":"Cancer science"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"馬場, 智久"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"向田, 直史"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously demonstrated that the proto-oncogene Pim-3 with serine/ threonine kinase activity was aberrantly expressed in cancer cells but not in the normal cells of the pancreas. In order to elucidate the molecular mechanism underlying aberrant Pim-3 expression in pancreatic cancer cells, we constructed luciferase expression vectors linked to 5′-flanking deletion mutants of the human Pim-3 gene and transfected human pancreatic cancer cells with the resultant vectors. The region up to -264 bp was essential for constitutive Pim-3 gene expression, and the mutation in the Ets-1 binding site (between -216 and -211 bp) reduced luciferase activities. Moreover, Ets-1 mRNA and protein were constitutively expressed together with Pim-3 in human pancreatic cancer cell lines. Chromatin immunoprecipitation assay demonstrated constitutive binding of Ets-1 to the 5′-flanking region of human Pim-3 gene between -249 and -183 bp. Pim-3 promoter activity and its protein expression were induced by transfection with wild type-Ets-1 and were reduced by transfection with dominant negative-Ets-1 or Ets-1 small-interfering RNA (siRNA). Furthermore, dominant negative-Ets-1 and Ets-1 siRNA reduced the amount of Bad phosphorylated at its Ser 112 and induced apoptosis, when they were transfected into human pancreatic cancer cells. Finally, Pim-3 cDNA transfection reversed Ets-1 siRNA-induced increase in apoptosis and decrease in Bad phosphorylation at its Ser 112. These observations would indicate that the transcription factor Ets-1 can induce aberrant Pim-3 expression and subsequently prevent apoptosis in human pancreatic cancer cells. © 2009 Japanese Cancer Association.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所がん病態制御","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00027545","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本癌学会 = Japanese Cancer Association / Wiley-Blackwell"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/j.1349-7006.2008.01059.x","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11808050","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-9032","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Li, Ying-Yi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wu, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuneyama, Koichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Baba, Tomohisa"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Mukaida, Naofumi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-MUKAIDA-N-396.pdf","filesize":[{"value":"541.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-MUKAIDA-N-396.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/27558/files/CA-PR-MUKAIDA-N-396.pdf"},"version_id":"c699b420-33b5-4c37-900b-c7fc371d01a8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Essential contribution of Ets-1 to constitutive Pim-3 expression in human pancreatic cancer cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Essential contribution of Ets-1 to constitutive Pim-3 expression in human pancreatic cancer cells."}]},"item_type_id":"4","owner":"3","path":["1779"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"27558","relation_version_is_last":true,"title":["Essential contribution of Ets-1 to constitutive Pim-3 expression in human pancreatic cancer cells."],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T11:06:45.533968+00:00"}