{"created":"2023-07-27T06:40:26.900637+00:00","id":29273,"links":{},"metadata":{"_buckets":{"deposit":"662088a2-ded2-4db0-b418-6f75a613b6cf"},"_deposit":{"created_by":3,"id":"29273","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"29273"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00029273","sets":["1979:1980:1981"]},"author_link":["50223","50216","50221","50222","50218","50217","15004","50220","50219"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"157","bibliographicPageStart":"147","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"Zoological Science"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In an investigation aimed at clarifying the mechanism of crystal dissolution of the calcium carbonate lattice in otoconia (the mineral particles embedded in the otolithic membrane) of the endolymphatic sac (ELS) of the bullfrog, cDNAs encoding the A- and E-subunits of bullfrog vacuolar proton-pumping ATPase (V-ATPase) were cloned and sequenced. The cDNA of the A-subunit consisted of an 11-bp 5′-untranslated region (UTR), a 1,854-bp open reading frame (ORF) encoding a protein comprising 617 amino acids with a calculated molecular mass of 68,168 Da, and a 248-bp 3′-UTR followed by a poly(A) tail. The cDNA of the E-subunit consisted of a 72-bp 5′-UTR, a 681-bp ORF encoding a protein of 226 amino acids with a calculated molecular mass of 26,020 Da, and a 799-bp 3′-UTR followed by a poly(A) tail. Western blot and immunofluorescence analyses using specific anti-peptide antisera against the V-ATPase A- and E-subunits revealed that these subunits were present in the ELS, urinary bladder, skin, testes, and kidneys. In the ELS, positive cells were scattered in the follicular epithelium which, as revealed by electron microscopy, corresponds to the location of mitochondria-rich cells. These findings suggest that V-ATPase, including the A- and E-subunits, exists in mitochondria-rich cells of the ELS, which might be involved in dissolution of the calcium carbonate crystals in the lumen of the ELS. © 2007 Zoological Society of Japan.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学環日本海域環境研究センター生物多様性研究部門","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本動物学会"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2108/zsj.24.147","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10545874","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0289-0003","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yajima, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kubota, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakakura, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasegawa, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katagiri, Nobuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomura, Hideaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasayama, Yuichi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Suzuki, Masakazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Shigeyasu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"SC-PR-SASAYAMA-Y-147.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-SASAYAMA-Y-147.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/29273/files/SC-PR-SASAYAMA-Y-147.pdf"},"version_id":"6dcdd373-3a59-4cf2-85c1-18663d3383ad"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning and expression of vacuolar proton-pumping ATPase subunits in the follicular epithelium of the bullfrog endolymphatic sac","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning and expression of vacuolar proton-pumping ATPase subunits in the follicular epithelium of the bullfrog endolymphatic sac"}]},"item_type_id":"4","owner":"3","path":["1981"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"29273","relation_version_is_last":true,"title":["Cloning and expression of vacuolar proton-pumping ATPase subunits in the follicular epithelium of the bullfrog endolymphatic sac"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-27T20:37:56.382043+00:00"}