{"created":"2023-07-27T06:40:31.131018+00:00","id":29369,"links":{},"metadata":{"_buckets":{"deposit":"d0fabea1-40ae-42da-aa38-5d212a9e3e53"},"_deposit":{"created_by":3,"id":"29369","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"29369"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00029369","sets":["1979:1980:1981"]},"author_link":["15029","69049","69052","69051","69055","69048","69054","26423","50519","69050","69053","69046","69047"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"300","bibliographicPageStart":"294","bibliographicVolumeNumber":"246","bibliographic_titles":[{"bibliographic_title":"General and Comparative Endocrinology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Calcitonin (CT) is a hormone that decreases serum calcium level by suppressing osteoclastic activity in the vertebrate bone. In vertebrates, the structure-function relationship of CTs has been studied extensively. We recently identified three CT superfamily peptides, Bf-CTFP1 to 3, and clarified the molecular and functional characteristics of their receptor and receptor activity-modifying protein in amphioxus, Branchiostoma floridae. However, the CT activity of Bf-CTFPs has yet to be investigated. In the present study, a functional analysis of Bf-CTFPs was performed using goldfish scales having both osteoclasts and osteoblasts. All Bf-CTFPs suppressed osteoclastic activity via a goldfish CT receptor. Although the primary amino acid sequences of the Bf-CTFPs showed low sequence similarity to vertebrate CTs, Bf-CTFP1 to 3 share three amino acids, Thr25, Thr27, and Pro32-NH2, that are required for receptor binding, with salmon CT. Moreover, homology model analysis revealed that the Bf-CTFPs form alpha-helical structures. The alpha-helical position and length of Bf-CTFP1 and 2 were conserved with those of a highly potent ligand, teleost CT. Interestingly, the composition of the alpha-helix of Bf-CTFP3 differed from those of teleost CT, despite that the action of Bf-CTFP3 on goldfish scales was the same as that of Bf-CTFP1 and 2. Collectively, the present study provides new insights into the structure-function relationship of CT and its functional evolution in chordates. © 2017 Elsevier Inc.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Embargo Period 12 months","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Academic Press Inc. / Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ygcen.2017.01.004","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/00166480","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © Elsevier (CC-BY NC ND)"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00654410","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0016-6480","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sekiguchi, Toshio"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Shiraishi, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satake, Honoo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuwasako, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Masayuki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Urata, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Shuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Endo, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikari, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hattori, Atsuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Srivastav, Ajai K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuo"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-16"}],"displaytype":"detail","filename":"OC-PR-SUZUKI-N-294.pdf","filesize":[{"value":"550.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"OC-PR-SUZUKI-N-294.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/29369/files/OC-PR-SUZUKI-N-294.pdf"},"version_id":"3872c419-46ef-42f4-b178-80c6821619ea"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Calcitonin-typical suppression of osteoclastic activity by amphioxus calcitonin superfamily peptides and insights into the evolutionary conservation and diversity of their structures","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Calcitonin-typical suppression of osteoclastic activity by amphioxus calcitonin superfamily peptides and insights into the evolutionary conservation and diversity of their structures"}]},"item_type_id":"4","owner":"3","path":["1981"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-02-27"},"publish_date":"2017-02-27","publish_status":"0","recid":"29369","relation_version_is_last":true,"title":["Calcitonin-typical suppression of osteoclastic activity by amphioxus calcitonin superfamily peptides and insights into the evolutionary conservation and diversity of their structures"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-27T17:45:28.645811+00:00"}