@article{oai:kanazawa-u.repo.nii.ac.jp:00029393,
 author = {関口, 俊男 and 佐藤, 政行 and 笹山, 雄一 and 北村, 敬一郎 and 渡辺, 良成 and 鈴木, 信雄 and Kase, Yoichi and Ikari, Takahiro and Sekiguchi, Toshio and Sato, Masayuki and Ogiso, Shouzo and Kawada, Tsuyoshi and Matsubara, Shin and Satake, Honoo and Sasayama, Yuichi and Endo, Masato and Kitamura, Kei-ichiro and Hattori, Atsuhiko and Watanabe, Takushi X. and Maruyama, Yusuke and Watanabe, Yoshinari and Funahashi, Hisayuki and Kambegawa, Akira and Suzuki, Nobuo},
 journal = {Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology},
 month = {Sep},
 note = {The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino-terminal cleavage peptide (N-proCT) (53 amino acids), CT (32 amino acids), and procalcitonin carboxyl-terminal cleavage peptide (C-proCT) (18 amino acids). As compared with C-proCT, N-proCT has been highly conserved among teleosts, reptiles, and birds, which suggests that N-proCT has some bioactivities. Therefore, both sardine N-proCT and sardine CT were synthesized, and their bioactivities for osteoblasts and osteoclasts were examined using our assay system with goldfish scales that consisted of osteoblasts and osteoclasts. As a result, sardine N-proCT (10− 7 M) activated osteoblastic marker enzyme activity, while sardine CT did not change. On the other hand, sardine CT (10− 9 to 10− 7 M) suppressed osteoclastic marker enzyme activity, although sardine N-proCT did not influence enzyme activity. Furthermore, the mRNA expressions of osteoblastic markers such as type 1 collagen and osteocalcin were also promoted by sardine N-proCT (10− 7 M) treatment; however, sardine CT did not influence their expressions. The osteoblastic effects of N-proCT lack agreement. In the present study, we can evaluate exactly the action for osteoblasts because our scale assay system is very sensitive and it is a co-culture system for osteoblasts and osteoclasts with calcified bone matrix. Both CT and N-proCT seem to influence osteoblasts and osteoclasts and promote bone formation by different actions in teleosts. © 2017 Elsevier Inc., Embargo Period 12 months},
 pages = {77--83},
 title = {Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish},
 volume = {211},
 year = {2017}
}