{"created":"2023-07-27T06:40:32.144720+00:00","id":29393,"links":{},"metadata":{"_buckets":{"deposit":"0f4b21dc-6453-4740-bae5-12541101c7ff"},"_deposit":{"created_by":3,"id":"29393","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"29393"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00029393","sets":["1979:1980:1981"]},"author_link":["69069","97427","69070","69071","69072","15029","69067","69066","69064","15004","69068","69065","86302","69063","69062","26423","69073","2213","2389","324","732","50519"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"83","bibliographicPageStart":"77","bibliographicVolumeNumber":"211","bibliographic_titles":[{"bibliographic_title":"Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"関口, 俊男"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"佐藤, 政行"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"笹山, 雄一"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"北村, 敬一郎"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"渡辺, 良成"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"鈴木, 信雄"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino-terminal cleavage peptide (N-proCT) (53 amino acids), CT (32 amino acids), and procalcitonin carboxyl-terminal cleavage peptide (C-proCT) (18 amino acids). As compared with C-proCT, N-proCT has been highly conserved among teleosts, reptiles, and birds, which suggests that N-proCT has some bioactivities. Therefore, both sardine N-proCT and sardine CT were synthesized, and their bioactivities for osteoblasts and osteoclasts were examined using our assay system with goldfish scales that consisted of osteoblasts and osteoclasts. As a result, sardine N-proCT (10− 7 M) activated osteoblastic marker enzyme activity, while sardine CT did not change. On the other hand, sardine CT (10− 9 to 10− 7 M) suppressed osteoclastic marker enzyme activity, although sardine N-proCT did not influence enzyme activity. Furthermore, the mRNA expressions of osteoblastic markers such as type 1 collagen and osteocalcin were also promoted by sardine N-proCT (10− 7 M) treatment; however, sardine CT did not influence their expressions. The osteoblastic effects of N-proCT lack agreement. In the present study, we can evaluate exactly the action for osteoblasts because our scale assay system is very sensitive and it is a co-culture system for osteoblasts and osteoclasts with calcified bone matrix. Both CT and N-proCT seem to influence osteoblasts and osteoclasts and promote bone formation by different actions in teleosts. © 2017 Elsevier Inc.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Embargo Period 12 months","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00029380","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.cbpa.2017.06.007","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/10956433","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © Elsevier (CC-BY NC ND)"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11183804","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1095-6433","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kase, Yoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikari, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sekiguchi, Toshio"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Sato, Masayuki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Ogiso, Shouzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawada, Tsuyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsubara, Shin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satake, Honoo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasayama, Yuichi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Endo, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitamura, Kei-ichiro"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Hattori, Atsuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Takushi X."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maruyama, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Yoshinari"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Funahashi, Hisayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kambegawa, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuo"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-02"}],"displaytype":"detail","filename":"OC-PR-SUZUKI-N-77.pdf","filesize":[{"value":"765.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"OC-PR-SUZUKI-N-77.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/29393/files/OC-PR-SUZUKI-N-77.pdf"},"version_id":"2369ecf3-1321-4a4d-a524-133acb13465b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish"}]},"item_type_id":"4","owner":"3","path":["1981"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-05"},"publish_date":"2017-12-05","publish_status":"0","recid":"29393","relation_version_is_last":true,"title":["Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T09:31:21.070208+00:00"}