{"created":"2023-07-27T06:44:18.353123+00:00","id":34485,"links":{},"metadata":{"_buckets":{"deposit":"36a4c61d-62de-471b-98f5-87a14a91a8f4"},"_deposit":{"created_by":3,"id":"34485","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"34485"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00034485","sets":["2812:2813:2819"]},"author_link":["80056","176"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-04-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2013-04-01 – 2015-03-31","bibliographic_titles":[{"bibliographic_title":"平成26(2014)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2014 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ミオグロビン（Mb）とミトコンドリア（mito）との直接的な関連性を解明するため、本萌芽的研究では、mitoに局在するMbに相互作用するタンパク質を同定することを目的に細胞生物学的解析を行った。マウス由来筋芽細胞株にFlagタグ付きMbを発現させるベクターを導入し、Mb過剰発現細胞を構築した。この細胞のmito画分からFlagを沈降し、Mbに相互作用しているタンパク質群を同定した。その結果、全体の約24%がmitoタンパク質であった。WBでもMbと呼吸鎖複合体や外膜のタンパク質との相互作用が確認された。以上の結果は、Mbがmitoへ相互作用し、mitoの機能修飾している可能性を示唆する。","subitem_description_type":"Abstract"},{"subitem_description":"Cellular respiration depends upon a coordinated response of the cardiovasculature and metabolism. Myoglobin (Mb), expressing specifically in myocytes, has been found in mitochondria. This unique finding has led us to the hypothesis that mitochondrial Mb may have a direct role for the regulation of mitochondrial respiration. In order to test this idea, we determined protein-protein interaction between Mb and mitochondrial proteins in genetically modified C2C12 cell by proteomic analysis, IP and WB. It was confirmed that Flag-tagged Mb localized in mitochondria in the C2C12 cells that express Flag-Mb stably. Proteomic analysis identified several mitochondrial proteins, which interacted with Mb. These proteins detected in IP samples of Mb-Flag, occupied 24% of entire proteins. IP and WB detected both outer and inner membrane proteins (VDAC and COX-IV) from the mitochondrial fraction. These results suggested the potential interaction of mitochondrial proteins with Mb in skeletal myocytes.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:25560336, 研究期間(年度):2013-04-01 – 2015-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「酸素輸送担体に作用する骨格筋ミトコンドリアタンパク質の網羅的探索」課題番号25560336\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25560336/25560336seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学人間社会研究域人間科学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00034472","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50323283"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50323283","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25560336/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25560336/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25560336/25560336seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25560336/25560336seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"HU-PR-MASUDA-K-kaken 2015-4p.pdf","filesize":[{"value":"575.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HU-PR-MASUDA-K-kaken 2015-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/34485/files/HU-PR-MASUDA-K-kaken 2015-4p.pdf"},"version_id":"9afb203d-c94d-4b4a-9c2b-5ed6895d9f27"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"酸素輸送担体に作用する骨格筋ミトコンドリアタンパク質の網羅的探索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酸素輸送担体に作用する骨格筋ミトコンドリアタンパク質の網羅的探索"},{"subitem_title":"Comprehensive analysis for interaction of oxgen binding protein with mitochondrial proteins in skeletal muscle","subitem_title_language":"en"}]},"item_type_id":"9","owner":"3","path":["2819"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"34485","relation_version_is_last":true,"title":["酸素輸送担体に作用する骨格筋ミトコンドリアタンパク質の網羅的探索"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:46:10.171281+00:00"}