{"created":"2023-07-27T06:44:19.888066+00:00","id":34519,"links":{},"metadata":{"_buckets":{"deposit":"18b7a0a9-89cd-4d08-b852-f7fb07d14f76"},"_deposit":{"created_by":3,"id":"34519","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"34519"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00034519","sets":["2812:2813:2820"]},"author_link":["84772","124"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-05-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2011-2013","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"MT1-MMPを発現抑制したHT1080細胞とRat1線維芽細胞の共培養では、FN繊維がRat1からHT1080細胞の細胞接着斑に伸展し、N-カドヘリン接着が形成された。また、N-カドヘリン阻害は、MT1-MMPを発現抑制したHT1080細胞におけるFN matrix形成を抑制した。以上の結果から、MT1-MMPは細胞接着斑上における初期のFNの重合を抑制することによりN-カドヘリン接着の安定化とFN matrix形成を阻害し、結果的に腫瘍細胞の細胞運動と増殖を誘導することが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"We have shown that membrane-type 1 matrix metalloproteinase (MT1-MMP) regulate fibronectin (FN) assembly by cleaving it, which results in promotion of cell motility and proliferation. FN matrix assembly requires the increased cytoskeletal tension generated by cadherin adhesions. In a co-culture of Rat1 fibroblasts and MT1-MMP-silenced but not control HT1080 cells, FN fibrils extended from Rat1 to cell-matrix adhesions in HT1080 cells, and N-cadherin adhesions were formed between these cells. MT1-MMP knockdown promoted FN matrix assembly and N-cadherin adhesions in HT1080 cells, which was abrogated by double knockdown with either integrin beta1 or FN. Conversely, inhibition of N-cadherin adhesions suppressed FN matrix formation in MT1-MMP-silenced cells. These data demonstrate that FN assembly initiated by MT1-MMP knockdown results in increased N-cadherin adhesions, which are prerequisite for further FN matrix formation.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23590356, 研究期間(年度):2011–2013","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「細胞外マトリックス分解と細胞運動の極性形成維持機構」課題番号23590356\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590356/23590356seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん進展制御研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00034506","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん進展制御研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40322119"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40322119","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590356/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590356/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590356/23590356seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590356/23590356seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-TAKINO-T-kaken 2014-5p.pdf","filesize":[{"value":"353.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-TAKINO-T-kaken 2014-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/34519/files/CA-PR-TAKINO-T-kaken 2014-5p.pdf"},"version_id":"684286dc-ce28-48e5-8a26-a06950f6971c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"細胞外マトリックス分解と細胞運動の極性形成維持機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"細胞外マトリックス分解と細胞運動の極性形成維持機構"},{"subitem_title":"A molecular mechanism that regulates extracellular matrix degration and cell polarity in migrating cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"3","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"34519","relation_version_is_last":true,"title":["細胞外マトリックス分解と細胞運動の極性形成維持機構"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T09:24:53.187614+00:00"}