{"created":"2023-07-27T06:44:25.057241+00:00","id":34633,"links":{},"metadata":{"_buckets":{"deposit":"2b74d1c0-dd57-46c3-84c8-8070e0acb851"},"_deposit":{"created_by":3,"id":"34633","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"34633"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00034633","sets":["2812:2813:2823"]},"author_link":["84772","2781"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-05-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2008-2010","bibliographic_titles":[{"bibliographic_title":"平成22(2010)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2010 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト扁平上皮癌細胞のコラーゲンゲル培養において、MT1-MMP活性を合成阻害剤やsiRNAを用いたknockdownnにより阻害すると細胞増殖が顕著に抑制された。この際、FAKとERKの活性化が抑制されていた。また、MT1-MMP阻害はインテグリンαvβ3を介したc-Srcの活性化も抑制することも見出した。c-Srcのエフェクター分子を検索したところ、コラーゲンゲル内においてMT1-MMPはPaxillinを介してERK活性化と細胞増殖を誘導することを証明した。MT1-MMPはコラーゲンゲル内において細胞外環境の変化を誘導し、c-Srcの活性化を惹起し、Paxillinを介してERK活性化と細胞増殖を誘導すると考えられた。","subitem_description_type":"Abstract"},{"subitem_description":"Membrane-type 1 matrix metalloproteinase (MT1-MMP) is essential for tumor invasion and growth. We show here that MT1-MMP induces extracellular signal-regulated kinase (ERK) activation in cancer cells cultured in collagen gel, which is indispensable for their proliferation. Inhibition of MT1-MMP by MMP inhibitor or small interfering RNA suppressed activation of focal adhesion kinase (FAK) and ERK in MT1-MMP-expressing cancer cells, which resulted in up-regulation of p21^<WAF1> and suppression of cell growth in collagen gel. Cell proliferation was also abrogated by the inhibitor against ERK pathway without affecting FAK phosphorylation. MT1-MMP and integrin α_vβ_3 were shown to be involved in c-Src activation, which induced FAK and ERK activation in collagen gel. These MT1-MMP-mediated signal transductions were paxillin dependent, as knockdown of paxillin reduced cell growth and ERK activation, and co-expression of MT1-MMP with paxillin induced ERK activation. The results suggest that MT1-MMP contributes to proliferation of cancer cells in the extracellular matrix by activating ERK through c-Src and paxillin.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:20590306, 研究期間(年度):2008–2010","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「細胞外微小環境変化と細胞運動誘導」課題番号20590306\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20590306/20590306seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん進展制御研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00034620","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40322119","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590306/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20590306/20590306seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-TAKINO-T-kaken 2011-5p.pdf","filesize":[{"value":"388.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-TAKINO-T-kaken 2011-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/34633/files/CA-PR-TAKINO-T-kaken 2011-5p.pdf"},"version_id":"a5308eaa-cbfe-4743-80ef-e66b6baf477c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"細胞外微小環境変化と細胞運動誘導","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"細胞外微小環境変化と細胞運動誘導"},{"subitem_title":"Cell migration induced by althernation of extracellular matrix microenvironment","subitem_title_language":"en"}]},"item_type_id":"9","owner":"3","path":["2823"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"34633","relation_version_is_last":true,"title":["細胞外微小環境変化と細胞運動誘導"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T13:19:34.939076+00:00"}