{"created":"2023-07-27T06:44:32.840367+00:00","id":34810,"links":{},"metadata":{"_buckets":{"deposit":"04423cf7-717c-434d-974e-49c28a0f33ca"},"_deposit":{"created_by":3,"id":"34810","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"34810"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00034810","sets":["2812:2813:2831"]},"author_link":["93784","97"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"9p.","bibliographicVolumeNumber":"2001-2002","bibliographic_titles":[{"bibliographic_title":"平成14(2002)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2002 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"週齢SCID mouse背部皮下両側に前立腺癌細胞株LNCaP 1.O×10^6を50%matrigelとともにそれぞれinoculate。腫瘍が増大したmouseから一方の腫瘍を摘出し、subline化した(LN-Pre)。その後、mouseをcastrationし、そのうち再び増大した腫瘍を摘出し、新たなsublineを樹立(LN-REC0)。LN-REC0をcastrationしたSCID mouseにinoculate。増大した腫瘍を摘出し、低アンドロゲン環境下においても腫瘍増殖を認める新しい前立腺癌細胞株(LN-REC4)を樹立した。この細胞株を用いてin vitro, in vivoにおいて、増殖速度、細胞特性につき検討した。新しく樹立した細胞株LN-REC4はin vitroではアンドロゲン感受性を保持していた。しかし、in vivoにおいては、castrationしたmouseでは造腫瘍能、増殖速度とも親株LNCaP、LN-Preと比較して速く、再燃前立腺癌の良いモデルであると考えられた。 また、同時に前立腺癌の再燃に関与する遺伝子として、可能性のあるものをmedline等で調べたところ、Cyclooxygenase-2(COX-2)の関与が考えられた。このため、COX-2の発現に乏しいLNCaP細胞でCOX-2を強制発現させ、COX-2の機能を調べた。COX-2はLNCaP細胞の増殖をin vitro, in vivoにおいても促進した。また、血管新生に関与する遺伝子のう右VEGFの発現を亢進させていた。","subitem_description_type":"Abstract"},{"subitem_description":"We inoculated LNCaP cells, androgen-sensitive human prostate cancer cells, onto 5-week aged scid mice subcutneously with 50 % matrigel. After tumor was increased visually, we castrated the mouse. First, tumor retarded for 2 weeks. Then it stated to enlarge. We sacrificed the mouse and took the tumor and subcultured. (LN-RECO). Next we inoculated the LN-RECO to the castrated mouse. Again we took the enlarged tumor and subcultured (LN-REC4).\nWe characterized LN-REC4 cells. Although LN-REC4 cells were stimulated by androgen in vitro, the growth speed of those cells in the castrated mouse was faster than LNCaP cells, suggesting that LN-REC4 seemed to be a good model that reflect the relapse of prostate cancer.\nWe also investigated the COX-2 gene since this gene seemed to be related with progression of prostate cancer. Overexpression of COX-2 gene in LNCaP cells induced cell proliferation in vitro and in vivo, and induced VEGF secretion.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:13671640, 研究期間(年度):2001–2002","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00034797","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医学部附属病院"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50248580","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671640/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-13671640/136716402002kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"ME-PR-MIZOKAMI-A-kaken 2003-9p.pdf","filesize":[{"value":"179.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-MIZOKAMI-A-kaken 2003-9p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/34810/files/ME-PR-MIZOKAMI-A-kaken 2003-9p.pdf"},"version_id":"3a58962e-7aef-45bf-b74b-c8d28c68d74e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"前立腺癌再燃時に関与する遺伝子の同定と遺伝子治療","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"前立腺癌再燃時に関与する遺伝子の同定と遺伝子治療"},{"subitem_title":"Identification of genes associated with relapse of prostate cancer and gene therapy","subitem_title_language":"en"}]},"item_type_id":"9","owner":"3","path":["2831"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"34810","relation_version_is_last":true,"title":["前立腺癌再燃時に関与する遺伝子の同定と遺伝子治療"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T14:41:29.166561+00:00"}