{"created":"2023-07-27T06:44:37.136633+00:00","id":34907,"links":{},"metadata":{"_buckets":{"deposit":"cec3ef10-f444-434d-ae9e-67f1ff6b7dd9"},"_deposit":{"created_by":3,"id":"34907","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"34907"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00034907","sets":["2812:2813:2839"]},"author_link":["23232","93773"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1993-1994","bibliographic_titles":[{"bibliographic_title":"平成6(1994)年度 科学研究費補助金 一般研究(C) 研究成果報告書"},{"bibliographic_title":"1994 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Epstein-Barr(EB)ウイルス初期遺伝子BZLF1にコードされるZタンパクは宿主細胞に潜伏感染したEBウイルスを複製サイクルに誘導する転写因子である。このZタンパクは、細胞性転写因子であるAP-1ファミリーとりわけFosタンパクとの間にそのアミノ酸配列において高い相同性を有する。ZタンパクとAP-1ファミリータンパクは共通のDNA配列を認識、結合するにもかかわらずZタンパクは、細胞性AP-1ファミリータンパクにより制御される細胞性遺伝子プロモーターを活性化することはできない。本研究ではZタンパクEBウイルスのプロモーター得意的活性化機構を検討するために、Fosタンパクとの融合タンパクを作成しEBウイルスのBMRF-1およびBHRF-1遺伝子プロモーターと細胞由来の92kDa-4型コラゲナーゼおよび組織特異的マトリックスメタロプロテイナーゼ阻害物質TIMP-1遺伝子プロモーターからの転写活性化能をZタンパクと比較検討した。ZタンパクによるEBウイルスプロモーター活性化には、その100-110アミノ酸領域、とりわけ102、104、105、108番目の4個のグルタミンが必須であり、また108番目のグルタミンを置換することによりZタンパクの活性化できなかった92kDa-4型コラゲナーゼ、TIMP-1遺伝子プロモーターを活性化できるようになった。一方ZのDNA結合領域N末端側に隣接する162-169アミノ酸領域をFosタンパクの対応する領域と置換することによってもそのプロモーター特異性を変化させることができた。Zタンパクの転写活性化能およびプロモーター特異性は、Zタンパクと二量体を形成しうる欠質変異体の発現によっても変化することからZタンパクの二量体としての立体構造により決定されていることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Epstein-Barr virus (EBV) immediate early gene BZFL1 encodes a transcription factor Z protein which induces EBV lytic cycle in latently infected cells. The Z protein shows an amino acid sequence homology with the members of cellular AP-1 family transcription factors, especially Fos proteins. Both Z proteins and AP-1 family members recognize and bind to the same DNA sequence, but Z cannot activate transcription from promotors of cellular genes which are stimulated by cellular AP-1 family members. In an attempt to analyze the mechanism of EBV promotor-specific transactivation by Z,chimeric proteins of Z and Fos were constructed and examined for their transactivation ability using EBV BMRF1, BHRF1, cellular 92-kDa type IV collagenase and Timp gene promotors as reporters. The region from amino acid 100 to 110 of Z,especially 4 gulutamine residues Gln 102,104,105 and 108 in this region, is essential for activation of viral promotors. Furthermore, Z proteins which had amino acid substitution of Gln 108 activated the transcription from 92-kDa type IV collagenase and Timp promotors which cannot be stimulated by the wild type Z.The promotor specificity of Z was also altered by substitution of the region from amino acid 162 to 169 adjacent to DNA binding domains with the corresponding region of Fos proteins. Formations of heterodimers of the wild type Z and chimeric proteins of deletion mutants affected transactivation ability and promotor specificity of them. These results suggest that the structure of dimer forms of Z proteins decides the transacitivation ability and promotor specificity.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:05670277, 研究期間(年度):1993–1994","subitem_description_type":"Other"},{"subitem_description":"出典：「EBウイルス転写調整因子Zタンパクのウイルスプロモーター選択的活性化機構の解析」研究成果報告書　課題番号05670277\n(KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00115239","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05670277/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-05670277/056702771994kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-05"}],"displaytype":"detail","filename":"CA-PR-SATO-H-kaken 1995-2p.pdf","filesize":[{"value":"41.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-SATO-H-kaken 1995-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/34907/files/CA-PR-SATO-H-kaken 1995-2p.pdf"},"version_id":"7efed667-b7e6-49bd-b396-563f19683f8a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"EBウイルス転写調整因子Zタンパクのウイルスプロモーター選択的活性化機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"EBウイルス転写調整因子Zタンパクのウイルスプロモーター選択的活性化機構の解析"},{"subitem_title":"The mechanism of Epstein-Barr Virus Promotor-specific transactivation by Z protein","subitem_title_language":"en"}]},"item_type_id":"9","owner":"3","path":["2839"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-05"},"publish_date":"2017-10-05","publish_status":"0","recid":"34907","relation_version_is_last":true,"title":["EBウイルス転写調整因子Zタンパクのウイルスプロモーター選択的活性化機構の解析"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T14:53:20.711717+00:00"}