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Studies on the molecular structure, organization, and expression of novel bombyxin genesof the silkmoth Bombyx mori
http://hdl.handle.net/2297/29509
http://hdl.handle.net/2297/295097de81b1c-c68d-4ce5-9d62-a687e8be1837
| 名前 / ファイル | ライセンス | アクション |
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TE-Thesis-Abu_Faiz_Md_Aslam-113p.pdf (972.7 kB)
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| アイテムタイプ | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2017-10-05 | |||||
| タイトル | ||||||
| タイトル | Studies on the molecular structure, organization, and expression of novel bombyxin genesof the silkmoth Bombyx mori | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者 |
Aslam, Abu Faiz Md.
× Aslam, Abu Faiz Md. |
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| 書誌情報 |
金沢大学大学院自然科学研究科 学位論文, 113p. 発行日 2010-07-01 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Insulin family peptide members play a pivotal role in regulation of growth, metabolism, and reproduction in vertebrates. Insulin-like peptides (ILPs) have also been identified from invertebrates, including several insect species. Bombyxin, isolated from the silkmoth Bombyx mori was the first ILP identified in insects. To gain insight into the role of bombyxin genes, the full genome of B. mori was analyzed, identified six novel bombyxin genes and grouped to five novel families: bombyxin-V through Z. All of these genes encoded preprobombyxin, the precursor molecule for bombyxin. Amino acid sequence comparison showed that these prepropeptides have 21 to 50% and 21 to 26% identities with previously characterized preprobombyxins and human preproinsulin, respectively. Genomic organization and chromosomal location of the genes were characterized. Each of bombyxin-V1 and -V2 had one intervening intron at 5' untranslated region (UTR), whereas Z1 had three introns - two at 5' UTR and one in C- peptide region. Human insulin and relaxin genes have intervening introns at almost the same position of these bombyxin genes. Reverse transcription-PCR and whole-mount in situ hybridization in different tissues and developmental stages were performed to observe temporal and spatial expression pattern. Newly identified bombyxin genes were expressed in diversified tissues. Bombyxin-V1, -W1 and -Y1 genes were predominantly expressed in four pairs of the medial neurosecretory cells of brain, which are the expression sites of other bombyxin genes also. The appearance of a short C-peptide, an extended A chain, appearance of high expression in non-feeding stages fat body, and induction upon starvation of bombyxin-X1 indicates its growth function and some structural similarities with insulin-like growth factors (IGFs). Bombyxin-Z1 was prejudiced to female insect. High level expression of Z1 in the follicular cells of ovary may suggest its function in reproduction. These results therefore demonstrate that bombyxin gene might have been originated from a vertebrate insulin-like common ancestral gene, and evolved into a diverse gene family with multiple functions. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位取得年月日：2010年9月, 取得学位：博士(理学), 授与大学：Division of Life Sciences, Kanazawa University, Japan(金沢大学), Chief supervisor Prof. Dr. Masafumi Iwami(岩見雅史) | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
