{"created":"2023-07-27T06:48:28.994855+00:00","id":40002,"links":{},"metadata":{"_buckets":{"deposit":"db1e0129-3575-4f27-97ba-0abc6bd8ef56"},"_deposit":{"created_by":3,"id":"40002","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"40002"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00040002","sets":["4162:4169:4188"]},"author_link":["366","63432","1779","63431","63430","63433","11946"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1276","bibliographicPageStart":"1270","bibliographicVolumeNumber":"101","bibliographic_titles":[{"bibliographic_title":"Biophysical Journal"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"淺川, 雅"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"福間, 剛士"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, we directly imaged subnanometer-scale structures of tubulins by performing frequency modulation atomic force microscopy (FM-AFM) in liquid. Individual α-helices at the surface of a tubulin protofilament were imaged as periodic corrugations with a spacing of 0.53 nm, which corresponds to the common pitch of an α-helix backbone (0.54 nm). The identification of individual α-helices allowed us to determine the orientation of the deposited tubulin protofilament. As a result, C-terminal domains of tubulins were identified as protrusions with a height of 0.4 nm from the surface of the tubulin. The imaging mechanism for the observed subnanometer-scale contrasts is discussed in relation to the possible structures of the C-terminal domains. Because the C-terminal domains are chemically modified to regulate the interactions between tubulins and other biomolecules (e.g., motor proteins and microtubule-associated proteins), detailed structural information on individual C-terminal domains is valuable for understanding such regulation mechanisms. The results obtained in this study demonstrate that FM-AFM is capable of visualizing the structural variation of tubulins with subnanometer resolution. This is an important first step toward using FM-AFM to analyze the functions of tubulins. © 2011 Biophysical Society.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノマテリアル研究所","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00039989","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Biophysical Society / Elsevier (Cell Press)"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bpj.2011.07.020","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.cell.com/biophysj/","subitem_relation_type_select":"URI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00566095","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-3495","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Asakawa, Hitoshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Ikegami, Koji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Setou, Mitsutoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukada, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuma, Takeshi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-06"}],"displaytype":"detail","filename":"FR-PR-ASAKAWA-H-1270.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"FR-PR-ASAKAWA-H-1270.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/40002/files/FR-PR-ASAKAWA-H-1270.pdf"},"version_id":"fd6664f9-045c-4c83-9ad3-6e69d3315f21"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid"}]},"item_type_id":"4","owner":"3","path":["4188"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-06"},"publish_date":"2017-10-06","publish_status":"0","recid":"40002","relation_version_is_last":true,"title":["Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:35:18.048267+00:00"}