{"created":"2023-07-27T06:50:06.055540+00:00","id":42579,"links":{},"metadata":{"_buckets":{"deposit":"9c23fe40-f39e-42ee-b0ab-05822c107fef"},"_deposit":{"created_by":18,"id":"42579","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"42579"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00042579","sets":["2812:2813:2827"]},"author_link":["20455","67961"],"item_10007_biblio_info_24":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicPageStart":"16p.","bibliographicVolumeNumber":"2005-2006","bibliographic_titles":[{"bibliographic_title":"平成18(2006)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2006 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-07","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"精子形成に関与する遺伝子や、抗ガン剤によって選択的に破壊される遺伝子情報を収集した結果、減数分裂に関与する遺伝子が候補遺伝子のひとつであることが明らかになった。減数分裂で最も重要な過程は相同染色体の正確な分配である。相同組み換えは、組み換えホットスポットと呼ばれる相同組み換えが起きやすい部位での一過的なDNA二本鎖切断(double strand breaks:DSB)によって開始されるとされる。DNA間の相同性の検索とその交叉反応において、中心的な役割を果たしているのが真核生物ではRad51やRad52,Rad54,Rad55,Rad57,DMC1,Rpa1などのタンパク質で群である。これらの遺伝子をノックアウトしたマウスでは、RAD51がDMC1の機能を相補できないことが報告され、DMC1の産物タンパクが相同染色体間の相同性のみを認識し対合させると考えられ、とくに減数分裂時に特異的な機能を持つ。本研究で明らかになったことは、DMC1(GenBank、NM_007068)の遺伝子産物についての検討である。DMC1は特発性不妊症患者で3つの転写物が存在することを明らかにし、これらの選択的スプライシングを受けた転写物が、精細胞系の制御を行っている。各選択的スプライシング産物であるイソフォームタンパクがモチーフやドメインが修飾された結果、精細胞のアポトーシスを誘導する可能性も考えられた。本遺伝子のスプライシングバリアントが減数分裂の制御に関与し、その結果精子形成能に影響を与えることを明らかにした。総括すると、精子形成不全のひとつの可能性として、DNA間の相補性を認識するという機構、すなわち相同染色体を認識の破綻により、減数分裂不全が生じ、精子形成不全が惹起される。DMC1はこれらの機構を説明できる、ひとつの候補遺伝子であることを明らかにした。","subitem_description_type":"Abstract"},{"subitem_description":"As a result of having retrieving genetic information from NCBI, a gene which was damaged selectively due to the various anti-cancer agents has been selected. Moreover recovery-related proteins after treating using anti-cancer agents were also associated with the genes during expressing in the meiotic stage. These candidate genes contributed to spermatogenesis and were naive to various anti-cancer agents depended on their dose. Generally speaking, the most important process of meiotic stage is accurate distribution of homologous chromosomes. It is assumed that the homologous recombination between chromosomes occurred initially in a transient DNA double-strand break (DSB) in the beginning to meiotic stage. In the cross reaction between homologous strands, the proteins such as Rad51 or Rad52, Rad54, Rad55, Rad57, DMC1, Rpa1 play a central role in a eukaryote. Specially, it is reported that RAD51 has not replaced the function of DMC1 (GenBank, NM_007068) in meiotic stage according to the knockout mouse. The transcripts of DMC1 are particularly specific in meiotic stage and enable to recognize only homogenous strands.\nOur study revealed that the transcripts of DMC1 were existed three products derived from alternative splicing. Clinically these transcripts had a various number in the idiopathic male infertility. According to analyzing the clinical samples, it was suggested that the number of the isoforms leaded to modify a motif and domain of the DMC1, and was thought to be induced apoptosis of spermatid. These genetic splicing variants contributed to meiotic control and clarified to affect the possibility of the spermatogenesis. Our study showed that one mechanism of spermatogenesis arrests was associated with the dysfunction of early stage during meiosis due to isofoms derived from domain specific proteins.","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:17591670, 研究期間(年度):2005-2006","subitem_description_type":"Other"},{"subitem_description":"研究機関: 金沢大学大学院医学系研究科","subitem_description_type":"Other"},{"subitem_description":"出典：「精細管内微小環境モジュレーションによる抗癌化学療法後精子形成回復救済療法の確立」研究成果報告書　課題番号17591670\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_10007_description_4":{"attribute_name":"研究代表者ID","attribute_value_mlt":[{"subitem_description":"90283134","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"0617591670","subitem_description_type":"Other"}]},"item_10007_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00048934","subitem_identifier_reg_type":"JaLC"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90283134"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90283134","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591670/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAENHI-PROJECT-17591670/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17591670/175916702006kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17591670/175916702006kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-11-16"}],"displaytype":"detail","filename":"ME-PR-KOH-E-kaken 2007-16p.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KOH-E-kaken 2007-16p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/42579/files/ME-PR-KOH-E-kaken 2007-16p.pdf"},"version_id":"d1a8e18e-5b4d-4a82-972d-dbd7839fe168"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子形成回復関連タンパクの同定","subitem_subject_scheme":"Other"},{"subitem_subject":"spermatogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"meiosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"alternative splicing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"homologuous chromosome","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高, 栄哲"}],"nameIdentifiers":[{"nameIdentifier":"67961","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90283134","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90283134"}]},{"creatorNames":[{"creatorName":"Koh, Eitetsu"}],"nameIdentifiers":[{"nameIdentifier":"20455","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90283134","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90283134"},{"nameIdentifier":"90283134","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090283134"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"精細管内微小環境モジュレーションによる抗癌化学療法後精子形成回復救済療法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"精細管内微小環境モジュレーションによる抗癌化学療法後精子形成回復救済療法の確立"},{"subitem_title":"Establishment of recovery spermatogenesis after chemotherapy due to the microenvironment modulation in seminiferous tubule","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"18","path":["2827"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-16"},"publish_date":"2017-11-16","publish_status":"0","recid":"42579","relation_version_is_last":true,"title":["精細管内微小環境モジュレーションによる抗癌化学療法後精子形成回復救済療法の確立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:34:35.215935+00:00"}