{"created":"2023-07-27T06:50:06.145457+00:00","id":42581,"links":{},"metadata":{"_buckets":{"deposit":"0eff3d71-6f3e-401d-a7c8-19caf22a2b37"},"_deposit":{"created_by":18,"id":"42581","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"42581"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00042581","sets":["2812:2813:2833"]},"author_link":["20455","67965"],"item_10007_biblio_info_24":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"1999-2000","bibliographic_titles":[{"bibliographic_title":"平成12(2000)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2000 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2001-03","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"前立腺癌細胞株(LNCaP,PC-3,DU145)を用いたステロイド代謝について、各ステロイドを基質にした薄層クロマトグラフィーによる分析結果、副腎ステロイドが、とりわけDHEAがステロイドプールとして大きく関与していることを平成11年度に明らかにした。また、アンドロゲン依存性前立腺癌株においてアンドロステンジオンからDHEAにいたる経路が優位に代謝しており、非依存性癌ではその逆の代謝活性を認めた。これらの現象の一部はステロイド代謝系におけるグルクロン酸抱合及び硫酸抱合がホルモン依存性に関与している可能性を示唆した。その代謝酵素のひとつであるglucuronosyltransferaseの発現の相違について明らかにし、その関与の可能性を検証した。これらの現象を分子生物学的に検討した結果、前立腺癌細胞株LNCaPにおいてはグルクロン酸抱合を触媒するglucuronosyltransferaseが強く発現しており、PC3,DU145において少ないことを見出した。平成12年度は、LNCaPに対してglucuronosyltransferaseの選択的なanti-sense oligomaやアンチセンスDNAを作製導入し、その増殖活性が抑制されるかどうか検討した。anti-sense oligoma導入し前立腺癌細胞の増額やステロイド代謝について検討したが、有意差をみとめなかったが、アンチセンスDNA導入によって、増殖活性の増加を抑制することを証明した。したがって、in vitro系であるが前立腺癌におけるアンドロゲン依存性は、アンドロゲン除去機構の低下がみられた結果であると結論できたが、その機構について今後の課題となった。","subitem_description_type":"Abstract"},{"subitem_description":"Adrenal androgens function as an androgen source within prostate and androgen target tissue. In this study, we compared the ability of three human prostatic cancer cell lines to metabolize the adrenal androgens, dehydroepiandrosterone (DHEA) and androstenedione in living cultured condition. Androgen-independent cell lines PC-3, DU145 and androgen-dependent cell line LNCaP were investigated. The effect of glucuronide and sulfate conjugates was also investigated. There is a strong tendency in PC-3 or DU145 to convert androstenedione to DHEA or DHEA-S reservoir. On the other hand, LNCaP is capable of converting DHEA into androstenedione and subsequently into dihydrotestosterone (DHT). Moreover, androgens were converted into a glucuronide conjugate in LNCaP, but not in PC-3 or DU145. As a result, the metabolism of the adrenal precursor shifted to androgen formation in LNCaP.This could be confirmed by means of reverse transcription-PCR of uridine diphospho-glucuronosyltransferase (UGT) 2B15. Kinetic properties of UGT activity in LNCaP revealed DHT to be a better substrate than testosterone for prostatic UGTs. In conclusion, our findings show that the adrenal precursor pool has the potential to contribute to the regulation of prostatic cells. Moreover, the presence of UGT activities in LNCaP may have a regulatory effect on the active androgen level in the intracellular environment.","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:11671542, 研究期間(年度):1999-2000","subitem_description_type":"Other"},{"subitem_description":"研究機関: 金沢大学医学部附属病院","subitem_description_type":"Other"},{"subitem_description":"出典：「前立腺癌細胞におけるステロイド代謝及びレセプター間のクロストーグに関する研究」研究成果報告書　課題番号11671542\n(KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_10007_description_4":{"attribute_name":"研究代表者ID","attribute_value_mlt":[{"subitem_description":"90283134","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"11671542","subitem_description_type":"Other"}]},"item_10007_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00048936","subitem_identifier_reg_type":"JaLC"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90283134"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90283134","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671542/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671542/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-11671542/116715422000kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-11671542/116715422000kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-11-16"}],"displaytype":"detail","filename":"ME-PR-KOH-E-kaken 2001-4p.pdf","filesize":[{"value":"120.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KOH-E-kaken 2001-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/42581/files/ME-PR-KOH-E-kaken 2001-4p.pdf"},"version_id":"7bb90002-4404-4594-9e88-5d50abec2e8a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"前立腺","subitem_subject_scheme":"Other"},{"subitem_subject":"癌","subitem_subject_scheme":"Other"},{"subitem_subject":"副賢ステロイド","subitem_subject_scheme":"Other"},{"subitem_subject":"グルクロン酸抱合","subitem_subject_scheme":"Other"},{"subitem_subject":"LNCaP","subitem_subject_scheme":"Other"},{"subitem_subject":"PC3","subitem_subject_scheme":"Other"},{"subitem_subject":"DU145","subitem_subject_scheme":"Other"},{"subitem_subject":"UGP","subitem_subject_scheme":"Other"},{"subitem_subject":"prostate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"adrenal steroid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glucuronide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"LNCaP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PC3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DU14","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"UGT","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高, 栄哲"}],"nameIdentifiers":[{"nameIdentifier":"67965","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90283134","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90283134"}]},{"creatorNames":[{"creatorName":"Koh, Eitetsu"}],"nameIdentifiers":[{"nameIdentifier":"20455","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90283134","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90283134"},{"nameIdentifier":"90283134","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090283134"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"前立腺癌細胞におけるステロイド代謝及びレセプター間のクロストーグに関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"前立腺癌細胞におけるステロイド代謝及びレセプター間のクロストーグに関する研究"},{"subitem_title":"Steroids metabolism and Cross-talk of steroid receptors in Prostatic cancer cells","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"18","path":["2833"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-16"},"publish_date":"2017-11-16","publish_status":"0","recid":"42581","relation_version_is_last":true,"title":["前立腺癌細胞におけるステロイド代謝及びレセプター間のクロストーグに関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:46:35.361483+00:00"}