{"created":"2023-07-27T06:50:13.085141+00:00","id":42913,"links":{},"metadata":{"_buckets":{"deposit":"141faee7-36d0-4812-98dc-f31d023120f5"},"_deposit":{"created_by":18,"id":"42913","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"42913"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00042913","sets":["2812:2813:2836"]},"author_link":["68628","68627"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"15p.","bibliographicVolumeNumber":"1996-1997","bibliographic_titles":[{"bibliographic_title":"平成9(1997)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"1997 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"今回の研究において私達は種々疾患の甲状腺組織を用いノーザンブロット法,in situ hybridization法および,モノクローナル抗体TSH受容体を用いた免疫組織化学的染色法を行いTSH受容体遺伝子のmRNAレベルと蛋白レベルにおける発現状態を検索し,種々の甲状腺疾患における甲状腺濾胞細胞でのTSH受容体発現と濾胞細胞の機能さらに甲状腺ホルモン全体におよぼす影響を検討した.\nA.材料.1996年から1997年にかけて金沢大学第2外科にて手術で得られた甲状腺組織を対象とした.組織は切除後直ちに凍結して-80℃に保存された.\nB.プローブと抗体.TSH受容体遺伝子の検索に用いたプローブはTSH receptor cDNA vectorである。免疫組織化学染色に用いた抗体はマウス抗TSH受容抗体であり,これらはParis South大学Dr.E.Milgromより供与された.\nC.結果\n1.種々の疾患の人甲状腺組織を用い、TSH受容体遺伝子のmRNA発現、蛋白質発現をnothernblot法,in situ hybridization法,免疫組織化学法用い検討した.\n2.これら3種の方法を用いたTSH受容体発現は各疾患別にみてほぼ一致して結果が得られた.\n3.バセドー病甲状腺ではmRNAレベル,蛋白レベル共に発現が高度に増加し,バセドー病甲状腺におけるTSH受容体のup-regulation機構が作動していることが推測された.\n4.非機能性の甲状腺腺腫,乳頭癌においてもTSH受容体はmRNAレベル,蛋白レベルで発現が増加していた.\n5.未分化癌のTSH受容体はmRNAレベル,蛋白レベル共に発現が認められなかった.","subitem_description_type":"Abstract"},{"subitem_description":"In the present study, we investigated TSH-receptor expression in hyman thyroids using immunohistochemistry, in situ hybridization and northern blot. The relationship between the TSH-receptor expression and the thyroid function were examined.\nA.Materials and Methoeds\nThe surgically resected thyroid tissues of various thyroid diseases were used in this study. The thyroid specimens were immediately frozed at -80C and stored. The TSH-receptor cDNA vector and the anti-human mouse monoclomnal antibody against TSH-receptor protein were used. These were kindly supplied by Dr.E.Milgrom (paris South University, France).\nB.Results\nThe obtained results were as follows :\n1. The immunohistochemical, in situ hybridization and northern blot study showed the same results in the expression of TSH-receptor in human thyroids of each case. examined.\n2. In Graves' thyroids, the elevated TSH-receotor expression was observed in both of protein level and mRNA level. This finding supported the theory that the up-regulation mechanism of TSH-receptor is one of the major causes of Graves' hyperthyroidism.\n3. In the thyroid adenomas and the carcinomas which are functionally inactive, the elevated TSH-receptor expression was observed in the protein level and mRNA level.\n4. In the undifferentiated thyroid carcinomas, The TSH-receptor expression was not detected in the protein level and mRNA level.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:08670196, 研究期間(年度):1996-1997","subitem_description_type":"Other"},{"subitem_description":"出典：「人TSH受容体発現のmRNAおよび蛋白レベルでの形態学的ならびに生化学的研究」研究成果報告書　課題番号08670196\n(KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00049262","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学保健学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60110540"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60110540","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670196/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670196/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-08670196/086701961997kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-08670196/086701961997kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-08"}],"displaytype":"detail","filename":"ME-PR-MIZUKAMI-Y-kaken 1998-15p.pdf","filesize":[{"value":"566.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-MIZUKAMI-Y-kaken 1998-15p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/42913/files/ME-PR-MIZUKAMI-Y-kaken 1998-15p.pdf"},"version_id":"660d112e-3cf3-4808-aea7-55b9ef9d6fa3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"人TSH受容体発現のmRNAおよび蛋白レベルでの形態学的ならびに生化学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"人TSH受容体発現のmRNAおよび蛋白レベルでの形態学的ならびに生化学的研究"},{"subitem_title":"Morphological and biochemical study of TSH-recetor expression","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2836"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-08"},"publish_date":"2017-12-08","publish_status":"0","recid":"42913","relation_version_is_last":true,"title":["人TSH受容体発現のmRNAおよび蛋白レベルでの形態学的ならびに生化学的研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:50:02.447896+00:00"}