{"created":"2023-07-27T06:50:26.632980+00:00","id":43256,"links":{},"metadata":{"_buckets":{"deposit":"42849092-6739-442a-8f26-0098aa569b46"},"_deposit":{"created_by":18,"id":"43256","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"43256"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00043256","sets":["4280:4292:4313"]},"author_link":["2828","190","69683","69775","73424","69773","73425","73422","69766","69777","69772","69708","73423"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-08-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"2143","bibliographicPageStart":"2131","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Cell Reports"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新明, 洋平"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"堀池, 俊秀"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細道, 一善"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"田嶋, 敦"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"河﨑, 洋志"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Folds in the cerebral cortex in mammals are believed to be key structures for accommodating increased cortical neurons in the cranial cavity. However, the mechanisms underlying cortical folding remain largely unknown, mainly because genetic manipulations for the gyrencephalic brain have been unavailable. By combining in utero electroporation and the CRISPR/Cas9 system, we succeeded in efficient gene knockout of Cdk5, which is mutated in some patients with classical lissencephaly, in the gyrencephalic brains of ferrets. We show that Cdk5 knockout in the ferret cerebral cortex markedly impaired cortical folding. Furthermore, the results obtained from the introduction of dominant-negative Cdk5 into specific cortical layers suggest that Cdk5 function in upper-layer neurons is more important for cortical folding than that in lower-layer neurons. Cdk5 inhibition induced severe migration defects in cortical neurons. Taken together, our findings suggest that the appropriate positioning of upper-layer neurons is critical for cortical folding. © 2017 The Author(s)","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系  ","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00049603","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.celrep.2017.08.024","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.elsevier.com/locate/issn/2211-1247"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/2211-1247","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2017 The Author(s)"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2211-1247","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shinmyo, Yohei"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Terashita, Yukari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Duong, Tung Anh Dinh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Horiike, Toshihide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawasumi, Muneo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hosomichi, Kazuyoshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Tajima, Atsushi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kawasaki, Hiroshi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-25"}],"displaytype":"detail","filename":"ME-PR-KAWASAKI-H-2131.pdf","filesize":[{"value":"6.5 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KAWASAKI-H-2131.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/43256/files/ME-PR-KAWASAKI-H-2131.pdf"},"version_id":"5d5e4af0-2c41-4244-9d66-c9fa0d2e74ff"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Folding of the Cerebral Cortex Requires Cdk5 in Upper-Layer Neurons in Gyrencephalic Mammals","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Folding of the Cerebral Cortex Requires Cdk5 in Upper-Layer Neurons in Gyrencephalic Mammals"}]},"item_type_id":"4","owner":"18","path":["4313"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-25"},"publish_date":"2017-12-25","publish_status":"0","recid":"43256","relation_version_is_last":true,"title":["Folding of the Cerebral Cortex Requires Cdk5 in Upper-Layer Neurons in Gyrencephalic Mammals"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T08:47:45.114117+00:00"}