@article{oai:kanazawa-u.repo.nii.ac.jp:00043489,
 author = {若山, 友彦 and 崔, 吉道 and 加藤, 将夫 and 辻, 彰 and 井関, 尚一 and Wakayama, Tomohiko and Sai, Yoshimichi and Ito, Akihiko and Kato, Yukio and Kurobo, Miho and Murakami, Yoshinori and Nakashima, Emi and Tsuji, Akira and Kitamura, Yukihiko and Iseki, Shoichi},
 issue = {6},
 journal = {Biology of Reproduction},
 month = {Jun},
 note = {The cell adhesion protein immunoglobulin superfamily 4A (IGSF4A) is expressed on the surfaces of spermatogenic cells in the mouse testis. During spermatogenesis, IGSF4A is considered to bind to the surface of Sertoli cells in a heterophilic manner. To identify this unknown partner of IGSF4A, we generated rat monoclonal antibodies against the membrane proteins of mouse Sertoli cells grown in primary culture. Using these monoclonal antibodies, we isolated a clone that immunostained Sertoli cells and reacted with the product of immunoprecipitation of the homogenate of mouse testis with anti-IGSF4A antibody. Subsequently, to identify the Sertoli cell membrane protein that is recognized by this monoclonal antibody, we performed expression cloning of a cDNA library from the mouse testis. As a result, we identified poliovirus receptor (PVR), which is another IGSF-type cell adhesion molecule, as the binding partner of IGSF4A. The antibodies raised against PVR and IGSF4A immunoprecipitated both antigens in the homogenate of mouse testis. Immunoreactivity for PVR was present in Sertoli cells but not in spermatogenic cells at all stages of spermatogenesis. Overexpression of PVR in TM4, a mouse Sertoli cell line, increased more than three-fold its capacity to adhere to Tera-2, which is a human cell line that expresses IGSF4A. These findings suggest that the heterophilic binding of PVR to IGSF4A is responsible, at least in part, for the interaction between Sertoli and spermatogenic cells during mouse spermatogenesis. © 2007 by the Society for the Study of Reproduction, Inc., Embargo Period 12 months, 金沢大学医薬保健研究域医学系},
 pages = {1081--1090},
 title = {Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and sertoli cells},
 volume = {76},
 year = {2007}
}