@article{oai:kanazawa-u.repo.nii.ac.jp:00043491,
 author = {太田, 哲生 and 沼田, 雅行 and 北川, 裕久 and 萱原, 正都 and 永川, 宅和 and 宮崎, 逸夫 and 山本, 美由紀 and 井関, 尚一 and Ohta, Tetsuo and Numata, Masayuki and Yagishita, H. and Futagami, Fumio and Tsukioka, Yuhji and Kitagawa, Hirohisa and Kayahara, Masato and Nagakawa, Takukazu and Miyazaki, Itsuo and Yamamoto, Miyuki and Iseki, Shoichi},
 issue = {12},
 journal = {British Journal of Cancer},
 month = {},
 note = {Recent studies have shown that bafilomycin A1-sensitive vacuolar-type H+-ATPase (V-ATPase) plays important roles in cell growth and differentiation. However, there is no published study that has focused on the expression of V-ATPase in human tumour tissues. This study was designed to examine the mRNA and protein levels for the 16 kilodalton (kDa) proteolipid of V-ATPase in human pancreatic carcinoma tissues. We first investigated the mRNA level for V-ATPase in six cases of invasive pancreatic cancers and two normal pancreases, using reverse transcription-polymerase chain reaction technique. Then, we examined immunohistochemically the level of V-ATPase protein in 49 pancreatic cancers and ten benign cystic neoplasms of the pancreas, using antisera raised against the 16 kDa proteolipid. There was a notable difference in the level of V-ATPase mRNA between normal and pancreatic carcinoma tissues, with no evident difference in the expression of the β-actin gene. Immunohistochemically, 42 out of 46 invasive ductal cancers (92%) displayed a mild to marked immunoreactivity for V-ATPase in the cytoplasm, whereas neither non-invasive ductal cancers nor benign cystic neoplasms expressed detectable immunoreactive proteins. These findings suggest that the overexpression of V-ATPase protein is characteristic of invasive pancreatic tumours. V-ATPase may play some crucial roles in tumour progression., Embargo Period 12 months, 金沢大学医薬保健研究域医学系},
 pages = {1511--1517},
 title = {Expression of 16 kDa proteolipid of vacuolar-tgpe H+-ATPase in human pancreatic cancer},
 volume = {73},
 year = {1996}
}