{"created":"2023-07-27T06:50:33.488133+00:00","id":43491,"links":{},"metadata":{"_buckets":{"deposit":"2c38008a-896b-4e12-9f69-3e25620e7c25"},"_deposit":{"created_by":18,"id":"43491","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"43491"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00043491","sets":["1132:1133:1134"]},"author_link":["71158","71177","71176","71159","71171","71178","72833","71155","71173","71175","71143","71170","72832","71157","71156","71172","71179","71174","72831"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"1517","bibliographicPageStart":"1511","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"British Journal of Cancer"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"太田, 哲生"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"沼田, 雅行"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"北川, 裕久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"萱原, 正都"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"永川, 宅和"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"宮崎, 逸夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山本, 美由紀"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"井関, 尚一"}],"nameIdentifiers":[{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recent studies have shown that bafilomycin A1-sensitive vacuolar-type H+-ATPase (V-ATPase) plays important roles in cell growth and differentiation. However, there is no published study that has focused on the expression of V-ATPase in human tumour tissues. This study was designed to examine the mRNA and protein levels for the 16 kilodalton (kDa) proteolipid of V-ATPase in human pancreatic carcinoma tissues. We first investigated the mRNA level for V-ATPase in six cases of invasive pancreatic cancers and two normal pancreases, using reverse transcription-polymerase chain reaction technique. Then, we examined immunohistochemically the level of V-ATPase protein in 49 pancreatic cancers and ten benign cystic neoplasms of the pancreas, using antisera raised against the 16 kDa proteolipid. There was a notable difference in the level of V-ATPase mRNA between normal and pancreatic carcinoma tissues, with no evident difference in the expression of the β-actin gene. Immunohistochemically, 42 out of 46 invasive ductal cancers (92%) displayed a mild to marked immunoreactivity for V-ATPase in the cytoplasm, whereas neither non-invasive ductal cancers nor benign cystic neoplasms expressed detectable immunoreactive proteins. These findings suggest that the overexpression of V-ATPase protein is characteristic of invasive pancreatic tumours. V-ATPase may play some crucial roles in tumour progression.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Embargo Period 12 months","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00049833","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cancer Research UK "}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 1996 Stockton Press."}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00574355","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0007-0920","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohta, Tetsuo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Numata, Masayuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yagishita, H."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Futagami, Fumio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukioka, Yuhji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitagawa, Hirohisa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kayahara, Masato"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Nagakawa, Takukazu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Miyazaki, Itsuo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yamamoto, Miyuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Iseki, Shoichi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-25"}],"displaytype":"detail","filename":"ME-PR-ISEKI-S-1511.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"ME-PR-ISEKI-S-1511.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/43491/files/ME-PR-ISEKI-S-1511.pdf"},"version_id":"544aec1c-ed95-4d4f-af79-c7eb6640fb06"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of 16 kDa proteolipid of vacuolar-tgpe H+-ATPase in human pancreatic cancer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of 16 kDa proteolipid of vacuolar-tgpe H+-ATPase in human pancreatic cancer"}]},"item_type_id":"4","owner":"18","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-01-25"},"publish_date":"2018-01-25","publish_status":"0","recid":"43491","relation_version_is_last":true,"title":["Expression of 16 kDa proteolipid of vacuolar-tgpe H+-ATPase in human pancreatic cancer"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T17:41:22.332802+00:00"}