@article{oai:kanazawa-u.repo.nii.ac.jp:00043498,
 author = {田島, 秀浩 and 太田, 哲生 and 岡本, 浩一 and 木下, 淳 and 古川, 裕之 and 牧野, 勇 and 林, 泰寛 and 中村, 慶史 and 尾山, 勝信 and 井口, 雅史 and 中川原, 寿俊 and 藤田, 秀人 and 高村, 博之 and 二宮, 致 and 藤村, 隆 and 若山, 友彦 and 井関, 尚一 and 清水, 康一 and Yoshimoto, Katsuhiro and Tajima, Hidehiro and Ohta, Tetsuo and Okamoto, Koichi and Sakai, Seisho and Kinoshita, Jun and Furukawa, Hiroyuki and Makino, Isamu and Hayashi, Hironori and Nakamura, Keishi and Oyama, Katsunobu and Inokuchi, Masafumi and Nakagawara, Hisatoshi and Itoh, Hiroshi and Fujita, Hideto and Takamura, Hiroyuki and Ninomiya, Itasu and Kitagawa, Hirohisa and Fushida, Sachio and Fujimura, Takashi and Wakayama, Tomohiko and Iseki, Shoichi and Shimizu, Koichi},
 issue = {3},
 journal = {Oncology Reports},
 month = {Sep},
 note = {Several recent studies have reported that selectins are produced during ischemia-reperfusion injury, and that selectin ligands play an important role in cell binding to the endothelium and in liver metastasis. Portal clamping during pancreaticoduodenectomy with vessel resection for pancreatic head cancer causes hepatic ischemia-reperfusion injury, which might promote liver metastasis. We investigated the liver colonization of pancreatic cancer cells under hepatic ischemia-reperfusion and examined the involvement of E-selectin and its ligands. A human pancreatic cancer cell line (Capan-1) was injected into the spleen of mice after hepatic ischemia-reperfusion (I/R group). In addition, to investigate the effect of an anti-E-selectin antibody on liver colonization in the IR group, mice received an intraperitoneal injection of the anti-E-selectin antibody following hepatic ischemia-reperfusion and tumor inoculation (IR+Ab group). Four weeks later, mice were sacrificed and the number of tumor nodules on the liver was compared to mice without hepatic ischemia-reperfusion (control group). The incidence of liver metastasis in the I/R group was significantly higher (16 of 20, 80%) than that in the control group (6 of 20, 30%) (P<0.01). Moreover, mice in the I/R group had significantly more tumor nodules compared to those in the control group (median, 9.9 vs. 2.7 nodules) (P<0.01). In the I/R+Ab group, only 2 of 5 (40%) mice developed liver metastases. RT-PCR and southern blotting of the liver extracts showed that the expression of IL-1 and E-selectin mRNA after hepatic ischemia-reperfusion was significantly higher than the basal levels. Hepatic ischemia-reperfusion increases liver metastases and E-selectin expression in pancreatic cancer. These results suggest that E-selectin produced due to hepatic ischemia-reperfusion is involved in liver metastasis., Embargo Period 6 months, 金沢大学医薬保健研究域医学系},
 pages = {791--796},
 title = {Increased E-selectin in hepatic ischemia-reperfusion injury mediates liver metastasis of pancreatic cancer},
 volume = {28},
 year = {2012}
}