{"created":"2023-07-27T06:50:35.583740+00:00","id":43558,"links":{},"metadata":{"_buckets":{"deposit":"f9b6d6d4-662a-44a9-bed0-b01b818d1d5b"},"_deposit":{"created_by":18,"id":"43558","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"43558"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00043558","sets":["2812:2813:2839"]},"author_link":["71331","71335"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"17p.","bibliographicVolumeNumber":"1993-1994","bibliographic_titles":[{"bibliographic_title":"平成6(1994)年度 科学研究費補助金 一般研究(C) 研究成果報告書"},{"bibliographic_title":"1994 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ダックB型肝炎ウィルス(DHBV)エンベロープ蛋白質(DHBVpreS)と特異的に結合するダック由来の新規カルボキシペプチダーゼgp180の全アミノ酸一次構造を推定できた。gp180は、1389個のアミノ酸からなる膜結合性糖蛋白質であり全アミノ酸配列を3分割するようにカルボキシペプチダーゼドメインを配列している。gp180全体のおよそ10%が細胞表面に局在する。\n作成した各種gp180欠失変異体とDHBVpreSとのbinding assayから、この結合ドメインはgp180C末端側、3番目のカルボキシペプチダーゼドメインにあることがわかった。また各ドメインの酵素活性については、現在検討中である。\nDHBV感染におけるgp180の機能を探るため、gp180導入細胞株を樹立しDHBV感染実験を行った。DHBV粒子はgp180発現細胞株ひよく吸着するが、培養14日後もDHBVの増殖は認められなかった。gp180は、DHBV感染の初期、細胞表面へのウィルス粒子の吸着に関わっているものと推察される。\nDHBV感染過程におけるgp180の機能、gp180の存在様式を明かにする目的で、抗gp180抗体を各種作成した。まだDHBVpreSとgp180の結合を阻害するような中和抗体は得ていないが、抗gp180抗体を使ったWestern解析、ニワトリgenomic DNAのSouthern解析からニワトリgp180の存在を示唆する結果を得た。gp180のカルボキシペプチダーゼドメインがtriplicateしたユニークな構造は、ダックに特異的なものではなく生物種を越えて普遍的に存在しているように思われる。また、抗gp180抗体を使ったWestern解析からgp180は、各ドメインがプロセッシングされることなく機能しているものと推察された。","subitem_description_type":"Abstract"},{"subitem_description":"We have described a 180 kDa host cell glycoprotein (gp 180) that bound DHBV particles. From the sequence of gp 180 cDNAs and genomic DNA,gp 180 consists of 1389 amino acids and is actually a head-to-tail array of three carboxypeptidase homology domains. Less than 10% of total gp 180 were localized on the cell surface.\nThe binding assay of gp 180 deletion mutants to DHBV envelope protein (preS) shows that preS binds on the third carboxypeptidase domain (domain C) of gp 180. The assay of carboxypeptidase activity of each domain is progressing.\nTo know the function of gp 180 on the entry pathway of DHBV,the cell lines expressing duck gp 190 were established by the transfection of plasmid DNA habroring gp 180 DNA and neo gene. DHBV particles adsorped on these cells. But we could not detect the proliferation of DHBV in the cells. These results indicate that gp 180 may act on the early atage of DHBV infection.\nTo know the function of gp 180 on the entry pathway of DHBV and the distribution of gp 180, anti-gp 180 antibodies were prepared from rabbits and mouse. These antibodies could not block the binding of gp 180 to DHBV preS.We are progressing to make the neutralizing antibodies of gp 180 to preS.We could not detect processed products of gp 180 by the western analysis using anti-gp 180 antibodies. gp 180 may act as an intact from. The presence of chicken gp 180 was determined by the western analysis and by the southen analysis. This fact suggests that unique structure of gp 180 is not specific to duck. gp 180 may widely distribute in vertebrates.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:05808056, 研究期間(年度):1993-1994","subitem_description_type":"Other"},{"subitem_description":"出典：「カルボキシペプチダーゼgp180の機能と構造」研究成果報告書　課題番号05808056\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00049900","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20178122"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20178122","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05808056/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05808056/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-05808056/058080561994kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-05808056/058080561994kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-29"}],"displaytype":"detail","filename":"CA-PR-KUROKI-K-kaken 1995-17p.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-KUROKI-K-kaken 1995-17p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/43558/files/CA-PR-KUROKI-K-kaken 1995-17p.pdf"},"version_id":"c841ac8e-d694-45d4-9c09-924029c516f3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"カルボキシペプチダーゼgp180の機能と構造","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"カルボキシペプチダーゼgp180の機能と構造"},{"subitem_title":"Function and Structure of Carboxypeptidase gp180","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2839"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-01-29"},"publish_date":"2018-01-29","publish_status":"0","recid":"43558","relation_version_is_last":true,"title":["カルボキシペプチダーゼgp180の機能と構造"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:53:12.867523+00:00"}