{"created":"2023-07-27T06:50:42.569529+00:00","id":43728,"links":{},"metadata":{"_buckets":{"deposit":"ee0968b9-8db3-4595-bd08-418c617ca8a4"},"_deposit":{"created_by":18,"id":"43728","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"43728"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00043728","sets":["2812:2813:2819"]},"author_link":["71929","71928"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05-26","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2012-04-01 – 2015-03-31","bibliographic_titles":[{"bibliographic_title":"平成26(2014)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2014 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"実験動物に強烈ストレスを負荷すると、PTSD類似の長期的異常行動とともに、脳内海馬においてミオシンVI(Myo6)の発現が著明に上昇した。胚性腫瘍細胞のP19細胞に、Myo6を強制導入すると細胞の増殖能力が低下したが、神経細胞やアストログリア細胞への分化能力には著変は見られなかった。一方、人工的にMyo6発現を抑制すると、P19細胞の増殖能力は亢進したが、分化能力は変化しなかった。生死に関わる強烈ストレスは、脳内Myo6発現を上昇させて、その結果神経幹細胞数が低下することが、PTSD様症状発症とその後の海馬萎縮に関与するかもしれない。Myo6を標的とするPTSD治療と予防対策が望まれる。","subitem_description_type":"Abstract"},{"subitem_description":"Severe fatal stress induced long-lasting bidirectional behavioral abnormalities relevant to posttraumatic stress disorder in adult mice, along with upregulation of the transcript and protein for myosin VI (Myo6) only in the hippocampus known to be enriched of proliferating neural progenitor cells. In embryonal carcinoma pluripotent P19 cells endowed to proliferate and differentiate into neuronal and astroglial lineages, stable overexpression of Myo6 attenuated the size and the activities of MTT reduction and BrdU incorporation in neurospheres clustered of proliferating cells. In these stable transfectants, no significant change was seen in the activity to commit and differentiate into neuronal and astroglial lineages. We succeeded in the generation of mice carrying a conditional Myo6 allele flanked by loxP sites, which are useful for generating mice detractive of Myo6 from a particular cell type for future investigation of the role of Myo6 in a variety of plasma cells.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:24650196, 研究期間(年度):2012-04-01 – 2015-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「逆向きモーター分子ミオシンVIのコンディショナルノックアウト表現型解析」課題番号24650196\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24650196/24650196seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050070","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50094454"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50094454","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24650196/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24650196/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24650196/24650196seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24650196/24650196seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-09"}],"displaytype":"detail","filename":"PH-PR-YONEDA-Y-kaken 2015-6p.pdf","filesize":[{"value":"311.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-YONEDA-Y-kaken 2015-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/43728/files/PH-PR-YONEDA-Y-kaken 2015-6p.pdf"},"version_id":"77f424a6-0b34-4c1e-8ac2-347bd5543a7e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"逆向きモーター分子ミオシンVIのコンディショナルノックアウト表現型解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"逆向きモーター分子ミオシンVIのコンディショナルノックアウト表現型解析"},{"subitem_title":"Phenotype analysis on conditional knockout mice defective of myosin VI","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2819"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-09"},"publish_date":"2018-02-09","publish_status":"0","recid":"43728","relation_version_is_last":true,"title":["逆向きモーター分子ミオシンVIのコンディショナルノックアウト表現型解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:45:03.039800+00:00"}