{"created":"2023-07-27T06:50:45.786229+00:00","id":43804,"links":{},"metadata":{"_buckets":{"deposit":"138be92c-1cd7-4e95-a83e-425808381cdc"},"_deposit":{"created_by":18,"id":"43804","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"43804"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00043804","sets":["2812:2813:2821"]},"author_link":["2451","88142"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-05-31","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2011-2012","bibliographic_titles":[{"bibliographic_title":"平成24(2012)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2012 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、3-D培養における上皮組織化や浸潤性獲得の分子基盤を明らかにすることを主たる目的とした。研究全体を通して以下の成果を得た。\n1) 乳腺上皮細胞のin vitro組織化において4倍体細胞の形成とその後の動態・増殖能を解析し、細胞増殖は核の倍数性に影響されないという結果を得た。\n2) 腫瘍細胞の3-D浸潤には細胞膜のMT1-MMPによって活性化されるMMP-2が必要であること、3-D培養で選択的におこるMMP-2の活性化にはMT1-MMPの細胞膜局在の増加によって引き起こされることを明らかにした。MMP-2の発現制御はエピジェネテイックに制御されており、その分子機構と標的遺伝子群を明らかにした。\n3) Metの発現の有無によって、乳腺上皮細胞や悪性黒色腫の分化転換を検出できる示唆を得た。分化転換の動態やその分子機構を明らかにする基盤を得た。","subitem_description_type":"Abstract"},{"subitem_description":"We aimed to elucidate molecular mechanisms of epithelial cell invasiveness and differentiation underlying epithelial cell morphogenesis. Our results are as follows.\n1) Mammary epithelial cell replication is not influenced by polyploidy.\n2) Activation of MMP-2 by MT1-MMP is required for 3-D invasiveness. Cell surface localization of MT1-MMP was depend on 3-D. MMP-2 expression was strongly correlated to invasiveness and regulated by epigenetics. We identified epigenetic factors and these target genes possibly involved in cancer malignancy.\n3) Cell surface Met was heterogeneously expressed in mammary epithelial cells and malignant melanomas. This might reflect invasive and malignant cell phenotypes.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23790221, 研究期間(年度):2011-2012","subitem_description_type":"Other"},{"subitem_description":"出典:研究課題「3-D上皮細胞組織化モデルにおける動的細胞分化転換と形態制御の研究」課題番号23790221\n(KAKEN:科学研究費助成事業データベース(国立情報学研究所))\n(https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23790221/23790221seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん進展制御研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050146","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん進展制御研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10523318"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10523318","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790221/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790221/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23790221/23790221seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23790221/23790221seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-16"}],"displaytype":"detail","filename":"CA-PR-SAKAI-K-kaken 2013-4p.pdf","filesize":[{"value":"447.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-SAKAI-K-kaken 2013-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/43804/files/CA-PR-SAKAI-K-kaken 2013-4p.pdf"},"version_id":"d0166a67-4d42-4d49-bb8b-6f72ae0ad5ff"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"3-D上皮細胞組織化モデルにおける動的細胞分化転換と形態制御の研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"3-D上皮細胞組織化モデルにおける動的細胞分化転換と形態制御の研究"},{"subitem_title":"Research of dynamic differentiation and morphogenesis in 3-D epithelial tubulogenesis","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2821"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-16"},"publish_date":"2018-02-16","publish_status":"0","recid":"43804","relation_version_is_last":true,"title":["3-D上皮細胞組織化モデルにおける動的細胞分化転換と形態制御の研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:42:38.866301+00:00"}