@article{oai:kanazawa-u.repo.nii.ac.jp:00043920,
 author = {小林, 淳二 and 野原, 淳 and 中嶋, 憲一 and 川尻, 剛照 and 稲津, 明広 and 山岸, 正和 and 小泉, 順二 and 馬渕, 宏 and Imamura, S. and Kobayashi, Junji and Sakasegawa, S. and Nohara, Atsushi and Nakajima, Kenichi and Kawashiri, Masa-aki and Inazu, Akihiro and Yamagishi, Masakazu and Koizumi, Junji and Mabuchi, Hiroshi},
 issue = {2},
 journal = {Journal of Lipid Research},
 month = {Feb},
 note = {The objective of this study was to establish a hepatic lipase (HL) assay method that can be applied to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women 45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at 546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-HCl (pH 9.5), 0.5 mM glycerol-1,2-dioleate, 0.4% (unless otherwise noted) polyoxyethylenenonylphenylether, 3 mM ATP, 3 mM MgCl2, 1.5 mM CaCl2, monoacylglycerol-specific lipase, glycerol kinase, glycerol-3-phosphate oxidase, 0.075% N,N-bis-(4-sulfobutyl)-3-methylaniline-2 Na, peroxidase, ascorbic acid oxidase. Reaction mixture R-2 contained 50 mM Tris-HCl (pH9.5), 0.15% 4-aminoantypirine. Automated assay for activity was performed with a Model 7080 Hitachi analyzer. In the lipase assay, 160 μl of R-1 was incubated at 37°C with 3 μl of samples for 5 min, and 80 μl of R-2 was added. Within-run coefficient of variations was 0.9-1.0%. Calibration curve of lipase activity was linear (r = 0.999) between 0 and 320 U/l. Analytical recoveries of purified HL added to plasma were 96.6-99.8%. HL activity in postheparin plasma measured in this method had a closer correlation with HL mass by a sandwich ELISA (r = 0.888, P , 0.0001) than those in the conventional method using [ 14C-]triolein (r = 0.730, P < 0.0001). This assay method for HL activity can be applied to an automatic clinical analyzer. Copyright © 2007 by the American Society for Biochemistry and Molecular Biology, Inc., 金沢大学医薬保健研究域医学系},
 pages = {453--457},
 title = {A novel method for measuring human hepatic lipase activity in postheparin plasma},
 volume = {48},
 year = {2007}
}