{"created":"2023-07-27T06:50:48.518024+00:00","id":43920,"links":{},"metadata":{"_buckets":{"deposit":"03ed8347-004b-4e13-87a1-834fe2d06677"},"_deposit":{"created_by":18,"id":"43920","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"43920"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00043920","sets":["1132:1133:1134"]},"author_link":["72634","72531","72536","70168","72534","68650","72537","72538","70167","72533","72577","72532","72530","69602","72633","72535","72539","69361"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"457","bibliographicPageStart":"453","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"Journal of Lipid Research"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 淳二"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野原, 淳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中嶋, 憲一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"川尻, 剛照"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"稲津, 明広"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"山岸, 正和"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"小泉, 順二"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"馬渕, 宏"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The objective of this study was to establish a hepatic lipase (HL) assay method that can be applied to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women 45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at 546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-HCl (pH 9.5), 0.5 mM glycerol-1,2-dioleate, 0.4% (unless otherwise noted) polyoxyethylenenonylphenylether, 3 mM ATP, 3 mM MgCl2, 1.5 mM CaCl2, monoacylglycerol-specific lipase, glycerol kinase, glycerol-3-phosphate oxidase, 0.075% N,N-bis-(4-sulfobutyl)-3-methylaniline-2 Na, peroxidase, ascorbic acid oxidase. Reaction mixture R-2 contained 50 mM Tris-HCl (pH9.5), 0.15% 4-aminoantypirine. Automated assay for activity was performed with a Model 7080 Hitachi analyzer. In the lipase assay, 160 μl of R-1 was incubated at 37°C with 3 μl of samples for 5 min, and 80 μl of R-2 was added. Within-run coefficient of variations was 0.9-1.0%. Calibration curve of lipase activity was linear (r = 0.999) between 0 and 320 U/l. Analytical recoveries of purified HL added to plasma were 96.6-99.8%. HL activity in postheparin plasma measured in this method had a closer correlation with HL mass by a sandwich ELISA (r = 0.888, P , 0.0001) than those in the conventional method using [ 14C-]triolein (r = 0.730, P < 0.0001). This assay method for HL activity can be applied to an automatic clinical analyzer. Copyright © 2007 by the American Society for Biochemistry and Molecular Biology, Inc.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050262","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology "}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1194/jlr.D600022-JLR200","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.jlr.org/content/48/2/453"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jlr.org/content/48/2/453","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © American Society for Biochemistry and Molecular Biology "}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00701215","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-2275","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Imamura, S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Junji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sakasegawa, S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nohara, Atsushi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Nakajima, Kenichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kawashiri, Masa-aki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Inazu, Akihiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yamagishi, Masakazu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Koizumi, Junji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Mabuchi, Hiroshi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-01"}],"displaytype":"detail","filename":"ME-PR-YAMAGISHI-M-453.pdf","filesize":[{"value":"205.5 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"ME-PR-YAMAGISHI-M-453.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/43920/files/ME-PR-YAMAGISHI-M-453.pdf"},"version_id":"949bdd82-3b96-4c7b-9bb7-ed39fc7e7678"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel method for measuring human hepatic lipase activity in postheparin plasma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel method for measuring human hepatic lipase activity in postheparin plasma"}]},"item_type_id":"4","owner":"18","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-01"},"publish_date":"2018-03-01","publish_status":"0","recid":"43920","relation_version_is_last":true,"title":["A novel method for measuring human hepatic lipase activity in postheparin plasma"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T17:41:38.427080+00:00"}