@article{oai:kanazawa-u.repo.nii.ac.jp:00044145, author = {酒井, 佳夫 and 餅田, 初音 and 吉田, 佳子 and 高村, 雅之 and 薄井, 荘一郎 and 和田, 隆志 and 本多, 政夫 and 金子, 周一 and Nasti, Alessandro and Sakai, Yoshio and Seki, Akihiro and Buffa, Geraldine Belen and Komura, Takuya and Mochida, Hatsune and Yamato, Masatoshi and Yoshida, Keiko and Ho, Tuyen T. B. and Takamura, Masayuki and Usui, Soichiro and Wada, Takashi and Honda, Masao and Kaneko, Shuichi}, issue = {12}, journal = {European Journal of Immunology}, month = {Dec}, note = {Stromal cells in adipose tissue are useful for repair/regenerative therapy as they harbor a substantial number of mesenchymal stem cells; therefore, freshly isolated autologous uncultured adipose tissue derived stromal cells (u-ADSCs) are useful for regenerative therapy, and obviate the need for mesenchymal stem cells. We evaluated the therapeutic effect of murine u-ADSCs and sorted subsets of u-ADSCs in a concanavalin A (ConA) induced murine model of hepatitis, as well as their characteristics. We found that 10–20% of u-ADSCs expressed the CD45 leukocyte-related antigen. CD68, which is a marker of macrophages (MΦs), was expressed by 50% of CD45+ u-ADSCs. About 90% of CD68+CD45+ cells expressed CD206 antigen, which is a marker of inhibitory M2-type MΦs. Genes related to M2-type MUs were especially more highly expressed by CD45+CD206+ u-ADSCs than by CD45− u-ADSCs. CD45+ u-ADSCs inhibited the expression of cytokines/chemokines and suppressed the proliferation of splenocytes stimulated with ConA. We observed that not only whole u-ADSCs, but also the CD45+ subset of u-ADSCs ameliorated the ConA-induced hepatitis in mice. In conclusion, we show that freshly isolated murine u-ADSCs were effective against acute hepatitis, and CD45+ u-ADSCs acting phenotypically and functionally like M2-type MΦs, contributed to the repair of liver tissue undergoing inflammation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Embargo Period 12 months, 金沢大学医薬保健研究域医学系}, pages = {2163--2174}, title = {The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune-inhibitory inflammatory cells}, volume = {47}, year = {2017} }