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Multivalency effects of hemagglutinin component of type B botulinum neurotoxin complex on epithelial barrier disruption
https://doi.org/10.24517/00050508
https://doi.org/10.24517/000505080aceb8ec-72b3-4054-960b-5de6fd649758
| 名前 / ファイル | ライセンス | アクション |
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ME-PR-AMATSU-S-80.pdf (8.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2018-04-16 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Multivalency effects of hemagglutinin component of type B botulinum neurotoxin complex on epithelial barrier disruption | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| ID登録 | ||||||||||||||
| ID登録 | 10.24517/00050508 | |||||||||||||
| ID登録タイプ | JaLC | |||||||||||||
| 著者 |
Amatsu, Sho
× Amatsu, Sho× Matsumura, Takuhiro× Yutani, Masahiro× Fujinaga, Yukako |
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| 著者別表示 |
阿松, 翔
× 阿松, 翔
× 松村, 拓大
× 油谷, 雅広
× 藤永, 由佳子
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| 提供者所属 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||||||||||
| 書誌情報 |
Microbiology and Immunology 巻 62, 号 2, p. 80-89, 発行日 2018-02-01 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0385-5600 | |||||||||||||
| NCID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA00738350 | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1111/1348-0421.12565 | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | 日本細菌学会 Japanese Society for Bacteriology / Wiley | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Hemagglutinin (HA) is one of the components of botulinum neurotoxin (BoNT) complexes and it promotes the absorption of BoNT through the intestinal epithelium by at least two specific mechanisms: cell surface attachment by carbohydrate binding, and epithelial barrier disruption by E-cadherin binding. It is known that HA forms a three-arm structure, in which each of three protomers has three carbohydrate-binding sites and one E-cadherin-binding site. A three-arm form of HA is considered to bind to these ligands simultaneously. In the present study, we investigated how the multivalency effect of HA influences its barrier-disrupting activity. We prepared type B full-length HA (three-arm form) and mini-HA, which is a deletion mutant lacking the trimer-forming domain. Size-exclusion chromatography analysis showed that mini-HA exists as dimers (two-arm form) and monomers (one-arm form), which are then separated. We examined the multivalency effect of HA on the barrier-disrupting activity, the E-cadherin-binding activity, and the attachment activity to the basolateral cell surface. Our results showed that HA initially attaches to the basal surface of Caco-2 cells by carbohydrate binding and then moves to the lateral cell surface, where the HA acts to disrupt the epithelial barrier. Our results showed that the multivalency effect of HA enhances the barrier-disrupting activity in Caco-2 cells. We found that basal cell surface attachment and binding ability to immobilized E-cadherin were enhanced by the multivalency effect of HA. These results suggest that at least these two factors induced by the multivalency effect of HA cause the enhancement of the barrier-disrupting activity. © 2017 The Societies and John Wiley & Sons Australia, Ltd | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Embargo Period 12 months | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | Copyright © 2017 The Societies and John Wiley & Sons Australia, Ltd | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 関連URI | ||||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/mandi1977/-char/ja/ | |||||||||||||
| 関連名称 | https://www.jstage.jst.go.jp/browse/mandi1977/-char/ja/ | |||||||||||||
