{"created":"2023-07-27T06:51:00.259195+00:00","id":44231,"links":{},"metadata":{"_buckets":{"deposit":"23a9b8aa-6b9a-4219-8298-0136a5ae30f7"},"_deposit":{"created_by":18,"id":"44231","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44231"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044231","sets":["2812:2813:2832"]},"author_link":["70288","49"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"11p.","bibliographicVolumeNumber":"1998-2001","bibliographic_titles":[{"bibliographic_title":"平成13(2001)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2001 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究を通して以下の研究成果を得た。\n1)マウス・メスの排卵後にIL-8の機能的ホモログであるマクロファージ炎症性蛋白(MIP)-2が、膣上皮で一過性に産生され、膣上皮への好中球浸潤を引き起すことを明らかにした。\n2)IgA腎症の急性型では尿中1L-8濃度が高く、尿中MCP-1濃度が検出感度以下であった。腎不全への移行が多い慢性型では、尿中MCP-1濃度が上昇しているのに対して、尿中IL-8濃'度の上昇は軽度であった。半月板形成性糸球体腎炎では、腎臓内で産生されるMIP-1αが半月板形成に、MCP-1が間質病変に関与していることを示唆する結果を得た。糖尿病腎症においては、間質のマクロファージがMCP-1陽性であり、尿中MCP-1濃度も上昇していた。\n3)IL-8遺伝子導入した癌細胞株を接種すると、血管新生と腫瘍形成が亢進した。癌病巣の低酸素状態の壊死近傍部位でIL-8蛋白・mRNAの発現が認められた。種々の癌細胞株を低酸素状態に暴露したところ、NF-κBとAP-1の2種類の転写因子が協調的に活性化され、IL-8遣伝子の転写が亢進していた。さらに、胃癌細胞株ではIL-8レセプターを発現していて、IL-8に反応して転移関連遺伝子の発現が誘導されていた。\n4)肝癌細胞株では、PEA3とAP-1とが協調的に恒常的に活性化されることによって、IL-8遺伝子転写が恒常的に起こっていた。C型肝炎ウイルス感染患者では、血清1L-8濃度のみが、慢性肝炎ならびに肝硬変患者に比べて、肝癌患者において上昇していた。さらにC型肝炎ウイルスのnonstructural protein(NS)5A蛋白遺伝子導入によって、IL-8遺伝子の転写と蛋白産生が誘導された。\n5)臍帯血中においてCD34陽性CCR1陰性細胞に赤芽球系の前駆細胞のほとんどが含まれることを明らかにした。","subitem_description_type":"Abstract"},{"subitem_description":"1) A functional interleukin-8 (IL-8) homologue, macrophage inflammatory protein (MIP)-2, was produced by mouse vaginal epithelium, periodically after ovulation and caused neutrophil infiltration into vaginal epithelium.\n2) In acute type of IgA nephropathy, urinary IL-8 levels were increased with undetectable urinary monocyte chemoattractant protein (MCP)-1. In contrast, MCP-1 but not IL-8 levels were increased in urines from chronic type of IgA nephropathy patients. Moreover, crescentic glomerulonephritis, MIP-lα and MCP-1 produced in the kidney, were responsible for crescent formation and interstitial lesions, respectively. Furthermore, in diabetic nephropathy, macrophages in interstitium were positive for MCP-1 and urinary MCP-1 levels were increased.\n3) IL-8 protein and mRNA were detected near necrotic areas inside tumor tissues, where oxygen content is presumed to be low. The exposure of several types of cell lines to hypoxia activated two transcription factors, NF-_kB and AP-1, and eventually induced IL-8 gene transcription. Human gastric cancer cell lines transfected with IL-8 gene exhibited faster tumor formation with enhanced angiogenesis when injected into nude mice, compared with parental cells. Moreover, most of gastric cancer cell lines expressed IL-8 receptors and IL-8 induced the expression of metastasis-related genes in some gastric cancer cell lines.\n4) In hepatoma cell lines, two distinct transcription factors, PEA3 and AP-1, were constitutively activated, which resulted in constitutive IL-8 gene transcription. Among patients infected with human hepatitis C virus, serum IL-8 levels were increased in patients suffering from hepatoma, when compared with chronic hepatits and liver cirrhosis patients. Moreover, the transduction of nonstructural protein (NS) 5A of human hepatitis C virus induced IL-8 gene transcription as well as IL-8 protein secretion.\n5) In cord blood, CCR1 expression among CD34-positive cells, was restricted to erythroid progenitor cells.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:10557249, 研究期間(年度):1998-2001","subitem_description_type":"Other"},{"subitem_description":"出典：「ケモカインならびにレセプターの分析法の開発とその病態検査学的意義の検討」研究成果報告書　課題番号10557249\n(KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050573","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん進展制御研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=30182067","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10557249/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-10557249/105572492001kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"CA-PR-MUKAIDA-N-kaken 2002-11p.pdf","filesize":[{"value":"744.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-MUKAIDA-N-kaken 2002-11p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44231/files/CA-PR-MUKAIDA-N-kaken 2002-11p.pdf"},"version_id":"983b7aac-45a2-41cb-81e9-f5ba1f1617fd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ケモカインならびにレセプターの分析法の開発とその病態検査学的意義の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ケモカインならびにレセプターの分析法の開発とその病態検査学的意義の検討"},{"subitem_title":"Development of assay systems for chemokines and their receptors and establishment of their diagnostic value","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2832"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-20"},"publish_date":"2018-04-20","publish_status":"0","recid":"44231","relation_version_is_last":true,"title":["ケモカインならびにレセプターの分析法の開発とその病態検査学的意義の検討"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:44:57.242059+00:00"}