{"created":"2023-07-27T06:51:01.726000+00:00","id":44272,"links":{},"metadata":{"_buckets":{"deposit":"91a78528-1472-446d-bd7a-f179a2f8d668"},"_deposit":{"created_by":18,"id":"44272","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44272"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044272","sets":["2812:2813:2829"]},"author_link":["74710","74711"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2002-2004","bibliographic_titles":[{"bibliographic_title":"平成16(2004)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2004 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tc-99m-Annexin V(Tc-AV)を用いてラット心筋虚血再灌流モデルにおけるアポトーシスの経時的変化を検討した。ウイスターラットにおいて左冠動脈(LCA)を20分(n=28)、15分(n=23)、10分(n=23)、5分(n=12)の間閉塞し、その後再灌流30、90分、6、24時間後にTc-Av(100-200MBq)を投与した。1時間後屠殺した。2核種オートラジオグラフィにてTc-AV(アポプトーシス)とT1(虚血範囲)の分布を画像化し解析した。5分閉塞群を除きTc-AVは不均一に再環流30、90分にて虚血部のmid myocardiumに集積し、6時間で心外膜、内膜側に拡大する傾向を示した。正常部に対する虚血部のTc-AVの集積はすべての再灌流時間において20分虚血で最も強く、15,10分では順次低下した。再環流30分での集積比は20,15 10,5分虚血群でそれぞれ7.36±2.95,4.46±3.16,2.02±0.47,0.97±0.08であった。再環流90分での集積比は20,15,10,5分虚血群でそれぞれ6.34±2.24,3.49±1.78,1.47±0.11,0.87±0.06であった。再環流6時間での集積比は20,15,10分虚血群でそれぞれ4.65±1.93,1.60±0.43,1.34±0.23であった。再環流24時間での集積比は20,15,10分虚血群でそれぞれ3.27±0.92,1.50±0.33,1.28±0.33であった。20分虚血再環流3,14日での集積比はそれぞれ1.84±0.55,1.65±0.31であった。20分虚血では明かな梗塞巣を認めたが、15分虚血では1%以下の梗塞病巣のみであり、10分以下の虚血では梗塞はなかった。以上より、アポトーシスは虚血再還流直後より生じ時間経過とともに低下してゆくことが示された。またそれは虚血の強度に依存して生じ、梗塞を起こさない虚血でもアポトーシスが生じていることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"To determine the time course, location and extent of this process in various severity of ischemia we studied ^<99m>Tc-annexin-V uptake in groups of rats following various intervals of coronary occlusion (20, 15, 10, 5 min) and reperfusion time (0.5, 1.5, 6, 24 hr, 3 day, 14 day). After reperfusion ^<99m>Tc-annexin-V was injected and 1 h later the rats were sacrificed. Dual tracer autoradiography was performed to assess ^<99m>Tc-annexin-V uptake and area at risk. In all 5-min occlusion and reperfusion models, no significant ^<99m>Tc-annexin V uptake was observed in the area at risk. In 10-min, 15-min, and 20-min ischemia and reperfusion models, significant ^<99m>Tc-annexin V uptake was observed depending on the severity of ischemia and reperfusion time. In each group of rats with the same reperfusion time, ^<99m>Tc-annexin V uptake depended on the severity of ischemia ; higher uptake in longer occlusion time. In each group of rats with the same ischemic time, ^<99m>Tc-annexin V uptake was highest in 0.5 h reperfusion model and the uptake decreased time dependently. In 20-min ischemia, a certain level of histological evidence of infarction was observed. In 15, 10, 5-min ischemia, there was no significant histological change was observed in 10-min and 5-min ischemia group. These data indicate that annexin binding depends on the severity of ischemia which results in the wide spectrum of histological change ; that is from infarction to no significant histological change. ^<99m>Tc-annexin V imaging may be feasible in the assessment of acute myocardial ischemia without infarction as well as infarction.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:14570842, 研究期間(年度):2002-2004","subitem_description_type":"Other"},{"subitem_description":"出典：「虚血心筋におけるプログラム細胞死、アポトーシスの画像化に関する核医学的研究」研究成果報告書　課題番号14570842\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050614","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学附属病院核医学診療科"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10251927"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10251927","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14570842/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14570842/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570842/145708422004kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570842/145708422004kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-26"}],"displaytype":"detail","filename":"ME-PR-TAKI-J-kaken 2005-6p.pdf","filesize":[{"value":"188.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TAKI-J-kaken 2005-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44272/files/ME-PR-TAKI-J-kaken 2005-6p.pdf"},"version_id":"fb2c2d19-71ea-481a-af89-ab7338d2bced"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"虚血心筋におけるプログラム細胞死、アポトーシスの画像化に関する核医学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"虚血心筋におけるプログラム細胞死、アポトーシスの画像化に関する核医学的研究"},{"subitem_title":"Scintigraphic imaging of programmed cell death, apoplcssis, in ischemic myocardium","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2829"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-26"},"publish_date":"2018-04-26","publish_status":"0","recid":"44272","relation_version_is_last":true,"title":["虚血心筋におけるプログラム細胞死、アポトーシスの画像化に関する核医学的研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:29:08.835871+00:00"}