{"created":"2023-07-27T06:51:02.417114+00:00","id":44287,"links":{},"metadata":{"_buckets":{"deposit":"21d8529b-eefd-4a6d-942b-b4c706706785"},"_deposit":{"created_by":18,"id":"44287","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44287"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044287","sets":["2812:2813:2818"]},"author_link":["74055","93"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-05-19","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2014-04-01 - 2016-03-31","bibliographic_titles":[{"bibliographic_title":"平成27(2015)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2015 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ゲノタイプ１ｂ型HCV 感染症例の肝細胞癌手術検体から癌幹細胞マーカーEpCAM陽性細胞分画を単離し、培養皿で継代が可能な細胞株（KH）を樹立した。KH細胞はJFH-1株やHJ3-5株のHCVの感染・複製を効率良く支持した。KH細胞はRIG-Iの変異を認めず、IFN誘導が保たれ、HCVの増殖は感染直後一端漸減し、再上昇を求めた。KH細胞はp53野生型であり、NOD-SCIDマウスに効率よく腫瘍を形成した。以上より、HCV感染・複製を支持する新規肝癌培養細胞株KH細胞を樹立した。KH細胞のゲノタイプ1bのHCV複製の確立には更なる検討が必要と考えられた。","subitem_description_type":"Abstract"},{"subitem_description":"We established a new cell line that is positive for cancer stem cell marker, EpCAM and supports efficient HCV replication and infection (JFH-1 and HJ3-5), from HCC tissue infected with genotype 1b HCV. KH cells have intact RIG-I sequence and produce IFN signaling, therefore, HCV replication once decline and increased again after infection. TP53 status of KH is wild and established tumor formation in NOD-SCID mice. Thus, we established a new HCC cell line that support efficient HCV replication and infection. Further studies should be needed to establish KH derived cells that support an efficient genotype 1b HCV replication.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26670378, 研究期間(年度):2014-04-01 - 2016-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「肝炎ウイルス複製を支持する新規肝癌由来培養細胞の樹立」課題番号26670378\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670378/26670378seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域保健学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050629","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00272980"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00272980","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670378/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670378/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670378/26670378seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670378/26670378seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-26"}],"displaytype":"detail","filename":"ME-PR-HONDA-M-kaken 2016-6p.pdf","filesize":[{"value":"116.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-HONDA-M-kaken 2016-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44287/files/ME-PR-HONDA-M-kaken 2016-6p.pdf"},"version_id":"36f2dd54-49e9-4fb4-8f82-a7b155b38626"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"肝炎ウイルス複製を支持する新規肝癌由来培養細胞の樹立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"肝炎ウイルス複製を支持する新規肝癌由来培養細胞の樹立"},{"subitem_title":"Establishment new HCC derived cell lines that support efficient hepatitis virus replication","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2818"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-26"},"publish_date":"2018-04-26","publish_status":"0","recid":"44287","relation_version_is_last":true,"title":["肝炎ウイルス複製を支持する新規肝癌由来培養細胞の樹立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:56:57.046797+00:00"}