{"created":"2023-07-27T06:51:05.349195+00:00","id":44354,"links":{},"metadata":{"_buckets":{"deposit":"9e38a719-678a-4add-93bd-613282a0e683"},"_deposit":{"created_by":18,"id":"44354","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44354"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044354","sets":["2812:2813:2826"]},"author_link":["75499","143"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"18p.","bibliographicVolumeNumber":"2006-2007","bibliographic_titles":[{"bibliographic_title":"平成19(2007)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2007 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々は形質細胞増殖因子β(TGF-β)と結合組織増殖因子(CTGF)という2つのサイトカインを新生マウス皮下に注入することによって確立した皮膚線維化モデルマウスにおける、抗CTGFマウスモノクロナル抗体による線維化の抑制を試みた。ヒトCTGFDNAを組み込んだベクターとジーンガンにてマウスに免疫するDNA免疫法により、CTGFmodule1〜4に対するモノクロナル抗体、17株の樹立に成功した。これら17株の抗CTGF中和活性についてスクリーニングを重ねたところ、抗CTGFmodule2に対する抗体の中和活性が最も強力であることが皮膚線維化モデルマウスにおいて示された。\n同抗体は、皮膚線維化モデルマウスに対して注入される前半のTGF-β及び後半のCTGFのいずれにおいても線維化を著明に抑制した。特に前半のTGF一β注入期においては、産生されたコラーゲンの蛋白量の50%、RT-PCRにおけるm-RNAの70%の抑制が得られた。以上のことは我々が得た抗ヒトCTGFモノクロナル抗体は、マウスの内因性のCTGFの活性をも抑制したことを意味する。\n今後は、皮膚以外においても、肝硬変モデル、腎硬化症モデル、肺線維症モデルなど他の線維化疾患の動物モデルにおいても、抗ヒトCTGFモノクロナル抗体の線維化抑制効果を検討するとともに、マウス抗ヒトCTGFモノクロナル抗体をヒト化し、各種ヒト線維化疾患、特に強皮症に対する新規治療薬確立への臨床応用の基盤を展開させたい。","subitem_description_type":"Abstract"},{"subitem_description":"We established skin fibrosis animal model by subcutaneous injection of two cytokines, TGF-β and CTGF. We have conducted this project to inhibit this fibrosis model by anti-CTGF antibody.\nWe succeeded in establishment of anti-human CTGF module 1, 2, 3 and 4 antibodies by DNA immunization method of human CTGF vector and gene gun. After the several screening studies, the anti-human CTGF module 2 antibody was revealed to be most potent of neutralizing activity.\nThe anti-human CTGF antibody inhibited skin fibrosis dramatically, both in the early phase of TGF-β injection and the late phase of CTGF injection. In this experiment, this anti-human CTGF antibody inhibited mouse internal CTGF action.\nIn the future, we are planning to create humanized anti-CTGF antibody for the clinical application.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18591240, 研究期間(年度):2006-2007","subitem_description_type":"Other"},{"subitem_description":"出典：「DNA免疫法による抗CTGF抗体のサイトカイン誘導皮膚線維化マウスモデルの抑制」研究成果報告書　課題番号18591240\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050696","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50142253"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50142253","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591240/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591240/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18591240/185912402007kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18591240/185912402007kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-11"}],"displaytype":"detail","filename":"ME-PR-TAKEHARA-K-kaken 2008-18p.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TAKEHARA-K-kaken 2008-18p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44354/files/ME-PR-TAKEHARA-K-kaken 2008-18p.pdf"},"version_id":"762d3a84-71b0-4b35-bffd-f2190d11a9ea"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"DNA免疫法による抗CTGF抗体のサイトカイン誘導皮膚線維化マウスモデルの抑制","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA免疫法による抗CTGF抗体のサイトカイン誘導皮膚線維化マウスモデルの抑制"},{"subitem_title":"The inhibition by anti-CTGF antibody produced by the DNA immunization method in the cytokine induced skin fibrosis model mice","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2826"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-11"},"publish_date":"2018-05-11","publish_status":"0","recid":"44354","relation_version_is_last":true,"title":["DNA免疫法による抗CTGF抗体のサイトカイン誘導皮膚線維化マウスモデルの抑制"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:24:19.694253+00:00"}