{"created":"2023-07-27T06:51:07.330654+00:00","id":44399,"links":{},"metadata":{"_buckets":{"deposit":"3840b66a-a69d-4f7f-9c93-e538c0032e7a"},"_deposit":{"created_by":18,"id":"44399","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44399"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044399","sets":["2812:2813:2827"]},"author_link":["75727","15643"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-05","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2004-2006","bibliographic_titles":[{"bibliographic_title":"平成18(2006)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2006 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"すべての細胞は外界からストレスを受けると、その恒常性を維持するために熱ショック応答を行なう。この生物に普遍的な応答機構は、熱ショックタンパク質(HSP : Heat Shock Protein)に担われている。多くのHSP遺伝子の発現(転写)は、そのプロモーター領域にある特異的塩基配列HSE(Heat Shock Element)に結合する熱ショック転写因子(HSF : Heat Shock Factor)により制御されている。一方酵母菌のHsf1は熱ショックによりリン酸化され転写活性化能力を3得する。本研究では「Hsf1のリン酸化と脱リン酸化の制御機構」について解析した。さらにストレス応答全般におけるHsf1の役割について明らかにするため、「さまざまなストレス応答反応におけるHsf1の役割」について検討した。\nDNAマイクロアレイ法により、酵母Hsf1は約70個の遺伝子の熱ショック転写に必要であり、その標的遺伝子は、HSPをコードするのみならず、熱ショック時の細胞壁の再構成や小胞体でのS-S結合の形成にも関与することを明らかにした。また、Hsf1のリン酸化はHsf1三量体が1つ結合するようなHSEを持つ遺伝子の転写には必要だが、2つ以上結合する場合は不要であることより、リン酸化はHsf1三量体間の相互作用と関連すること、Hsf1のリン酸化には三量体形成が必須であり、また、DNA結合領域の構造変化と密接な関連があること、DNA結合領域の構造変化は転写活性化能力を調節することを明らかにした。さらに、ヒトのHSF1は酵母のHsf1と異なる機構によりHSEに結合する結果も得た。\n今後は、さまざまなHSE配列は遺伝子特異的な転写調節、さらに、ストレス特異的な制御について解析していく必要がある。","subitem_description_type":"Abstract"},{"subitem_description":"All organisms respond to elevated temperatures by changing transcription programs and expressing a set of proteins called heat shock proteins (HSPs). In eukaryotes, heat sock transcription factor (HSF) activates transcription of HSP genes. The target genes of HSF contain a cis-acting sequence designated the heat shock element. The baker's yeast, Saccharomyces cerevisiae, has one HSF encoded by the HSF1 locus. Under heat shock condition, Hsfl protein is extensively phosphorylated and acquires a stronger activating ability, although detailed regulatory mechanisms remain unknown. In this project, I focused on mechanisms of Hsfl phosphorylation and dephosphorylation' and 'roles of Hsfl in the response to various stresses'.\nBy using DNA microarray analysis, we have shown that yeast Hsfl is necessary for heat-induced transcription of〜70 genes and that target genes encode molecular chaperones and proteins involved in a broad range biological functions. We have characterized multicopy suppressor genes of a temperature-sensitive hsfl mutation and found that Hsfl in concert with a protein kinase Pkc1 regulates cell wall remodeling in response to heat shock. Heat-induced hyperphosphorylation of Hsfl was required for transcriptional activation of approximately half of Hsfl target genes. This requirement was related to cooperative interactions among Hsfl trimers bound to the HSE. Consistent with this, oligomerization of Hsfl was prerequisite for stress-induced hyperphosphorylation of Hsfl. We have also shown that human HSF1 recognizes HSEs in a slightly different way than yeast Hsfl.\nTaken together, these results suggest that the architecture of the HSE is an important determinant for HSF-HSE interactions.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16570142, 研究期間(年度):2004-2006","subitem_description_type":"Other"},{"subitem_description":"出典：「ストレス応答における熱ショック転写因子のリン酸化制御」研究成果報告書　課題番号16570142\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050741","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域保健学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00225848"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00225848","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16570142/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16570142/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16570142/165701422006kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16570142/165701422006kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-17"}],"displaytype":"detail","filename":"ME-PR-SAKURAI-H-kaken 2007-5p.pdf","filesize":[{"value":"212.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SAKURAI-H-kaken 2007-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44399/files/ME-PR-SAKURAI-H-kaken 2007-5p.pdf"},"version_id":"ce24a36c-436c-4c0c-82cc-ca2dfc3cab78"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ストレス応答における熱ショック転写因子のリン酸化制御","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ストレス応答における熱ショック転写因子のリン酸化制御"},{"subitem_title":"Phosphorylation of heat shock transcription factor in the stress response","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2827"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-17"},"publish_date":"2018-05-17","publish_status":"0","recid":"44399","relation_version_is_last":true,"title":["ストレス応答における熱ショック転写因子のリン酸化制御"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:34:14.818583+00:00"}